Nucleoid-associated proteins (NAPs) maintain bacterial nucleoid configuration through their architectural properties of DNA bending, wrapping, and bridging. However, the contribution of DNA structural alterations to DNA-NAP recognition at the genomic scale remains unresolved. Present work dissects the DNA sequence, shape and altered structural preferences at a genomic scale for six NAPs in Mycobacterium tuberculosis. Results suggest narrower minor groove width (MGW) and higher DNA rigidity are marked for the binding sites of EspR and Lsr2, while mIHF, MtHU and NapM have heterogeneous DNA structural predilections. In contrast, WhiB4–DNA-binding sites were characterized by wider MGW, highly deformable and less curved DNA. This work provides systematic insight into NAP-mediated genome organization as a function of DNA structural features.

中文翻译：

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类核蛋白结合区的分析揭示了影响结核分枝杆菌基因组组织的 DNA 结构特征

类核蛋白 (NAP) 通过其 DNA 弯曲、包裹和桥接的结构特性来维持细菌类核构型。然而，DNA 结构改变对基因组规模的 DNA-NAP 识别的贡献仍未得到解决。目前的工作在基因组规模上剖析了结核分枝杆菌中六个 NAP 的 DNA 序列、形状和改变的结构偏好. 结果表明，EspR 和 Lsr2 的结合位点标记了更窄的小沟宽度 (MGW) 和更高的 DNA 刚性，而 mIHF、MtHU 和 NapM 具有异质 DNA 结构偏好。相比之下，WhiB4-DNA 结合位点的特点是更宽的 MGW、高度可变形和更少弯曲的 DNA。这项工作提供了对 NAP 介导的基因组组织作为 DNA 结构特征的函数的系统洞察。