Electrochemically active bacteria (EAB) have attracted considerable attention in various fields, such as wastewater treatment, energy recovery, and biogeochemistry. However, a rapid, simple, and precise method for the selection of EAB remains a challenge because of the lack of sensitive probes and rapid analytical methods. To solve this problem, riboflavin was adapted as a fluorescent probe and a program was designed to evaluate the extracellular electron transfer (EET) ability using kinetic fluorescence data. With this method, the EET abilities of 1152 strains isolated from environmental sources, including a municipal wastewater treatment plant, freshwater lake sediments, and submarine sediments, were tested, and 49 strains were identified as EAB. Hence, the results provide new insights into the abundance and roles of strains of EAB in different environmental and engineered water samples. Taken together, we developed a simple and practical method for quantitative evaluation of the EET ability of microbes and further rapid screening of EAB from environments.

中文翻译：

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用于高通量筛选电化学活性细菌的基于平板的动力学荧光测试

电化学活性细菌（EAB）在废水处理、能源回收和生物地球化学等各个领域引起了广泛关注。然而，由于缺乏灵敏的探针和快速的分析方法，用于选择 EAB 的快速、简单和精确的方法仍然是一个挑战。为了解决这个问题，核黄素被改编为荧光探针，并设计了一个程序来使用动力学荧光数据评估细胞外电子转移 (EET) 能力。该方法对从市政污水处理厂、淡水湖沉积物和海底沉积物等环境来源分离的1152株菌株的EET能力进行了测试，其中49株被鉴定为EAB。因此，结果为 EAB 菌株在不同环境和工程水样中的丰度和作用提供了新的见解。总之，我们开发了一种简单实用的方法来定量评估微生物的 EET 能力，并进一步从环境中快速筛选 EAB。