ABSTRACT

The current study aims to explore the antimicrobial and anticancer activity of biogenic silver nanoparticles (B-AgNPs) synthesized using Bael leaf extract. AgNPs nanoparticles characterized using FESEM, TEM, XRD, and UV-Spectrophotometry. TEM analysis revealed that B-AgNPs were predominantly spheroidal with particle size distribution in the range of 7-10 nm. In MTT assay, when B-AgNPs were applied in colon cancer (HCT-116) cells and human colon epithelial cell line (FHC) cells. B-AgNPs exhibited specific cytotoxicity to HCT-116 as minimal effect observed in normal FHC cells. Moreover, the fluorescence microscopic examination after nucleus staining as well as DNA fragmentation also confirmed AgNPs spectific cytotoxicity towards HCT-116. Further, AgNPs demonstrated excellent antibiofilm and antibacterial activity in both Gram-Positive and Gram-negative bacteria. Hence, overall, we categorically concluded that the biogenic silver nanoparticles synthesized using Bael leaf extract possess multiple biological activities and might be possibly used as multipurpose therapeutic material with no or little systemic toxicity.

摘要

目前的研究旨在探索使用 Bael 叶提取物合成的生物银纳米粒子 (B-AgNPs) 的抗菌和抗癌活性。使用 FESEM、TEM、XRD 和紫外分光光度法表征 AgNPs 纳米颗粒。TEM 分析显示 B-AgNPs 主要呈球形，粒径分布在 7-10 nm 范围内。在MTT测定中，当B-AgNPs应用于结肠癌（HCT-116）细胞和人结肠上皮细胞系（FHC）细胞时。B-AgNPs 对 HCT-116 表现出特异性细胞毒性，因为在正常 FHC 细胞中观察到的影响最小。此外，核染色后的荧光显微镜检查以及 DNA 片段化也证实了 AgNPs 对 HCT-116 的特异性细胞毒性。更远，AgNPs 在革兰氏阳性菌和革兰氏阴性菌中均表现出优异的抗生物膜和抗菌活性。因此，总体而言，我们断然得出结论，使用 Bael 叶提取物合成的生物银纳米颗粒具有多种生物活性，可能用作多用途治疗材料，没有或几乎没有全身毒性。