Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor arrays.

中文翻译：

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在纳米孔阵列上多路直接检测条形码蛋白报告基因

使用纳米孔检测特定蛋白质目前具有挑战性。为了应对这一挑战，我们开发了一组超过 20 个纳米孔可寻址蛋白质标签，这些标签被设计为报告基因（NanoporeTERs，或 NTERs）。NTER 由分泌标签、折叠结构域和靶向纳米孔的 C 末端尾部构成，其中可以编码任意肽条形码。我们展示了使用 MinION 纳米孔传感器阵列同时检测多达 9 个在细菌或人体细胞中表达的 NTER。