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Treatment of acid rock drainage using a sulphate-reducing bioreactor with a limestone precolumn
Environmental Technology ( IF 2.8 ) Pub Date : 2021-08-20 , DOI: 10.1080/09593330.2021.1968039
Gabriela Méndez 1, 2 , Gabriel Trueba 1 , Reyes Sierra-Alvarez 3 , Valeria Ochoa-Herrera 1, 2, 4
Affiliation  

ABSTRACT

Sulphate reducing bacteria (SRB) offer promise for the treatment of mine waste due to their effectiveness removing toxic heavy metals as highly insoluble metal sulphides and their ability to generate alkalinity. The main objective of this study was to develop a treatment composed of a sulphate-reducing bioreactor with a limestone precolumn for the removal of Cu(II) from a synthetic ARD. The purpose of the limestone column was to increase the pH values and decrease the level of Cu in the effluent to prevent SRB inhibition. The system was fed with a pH-2.7 synthetic ARD containing Cu(II) (10–40 mg/L), sulphate (2000 mg/L) and acetate (2.5 g COD/L) for 150 days. Copper removal efficiencies in the two-stage system were very high (95–99%), with a final concentration of 0.53 mg/L Cu, and almost complete removal occurred in the limestone precolumn. In the same manner, the acidity of the synthetic ARD was effectively reduced in the limestone precolumn to 7.3 and the pH was raised in the bioreactor (7.3–8.0). COD consumption by methanogens was predominant from day 0–118, but SRB dominated at the end of the experiment (day 150) when the average COD removal and sulphide production were 74.8% and 61.7%, respectively. Study of the microbial taxonomic composition in the bioreactor revealed that Methanosarcina and Methanosaeta were the most prevalent methanogens while the genera Desulfotomaculum and Syntrophobacter were the dominant SRB. Among the SRB identified Desulfotomaculum intricatum (99% identity) and Desulfotomaculum acetoxidans (96%) were the most abundant sequences of bacteria capable of using acetate.



中文翻译:

使用带石灰石预柱的硫酸盐还原生物反应器处理酸性岩石废水

摘要

硫酸盐还原菌 (SRB) 可以有效地去除有毒重金属作为高度不溶性金属硫化物,并且能够产生碱度,因此为处理矿山废物提供了希望。本研究的主要目的是开发一种由硫酸盐还原生物反应器和石灰石预柱组成的处理方法,用于从合成 ARD 中去除 Cu(II)。石灰石柱的目的是提高 pH 值并降低流出物中的 Cu 水平,以防止 SRB 抑制。向系统中加入含有 Cu(II) (10–40 mg/L)、硫酸盐 (2000 mg/L) 和乙酸盐 (2.5 g COD/L) 的 pH-2.7 合成 ARD,持续 150 天。两级系统中的铜去除效率非常高 (95–99%),铜的最终浓度为 0.53 mg/L,并且在石灰石预柱中几乎完全去除。以同样的方式,合成 ARD 的酸度在石灰石预柱中有效降低至 7.3,并且生物反应器中的 pH 值升高(7.3–8.0)。从第 0 天到第 118 天,产甲烷菌的 COD 消耗占主导地位,但 SRB 在实验结束时(第 150 天)占主导地位,此时平均 COD 去除率和硫化物产量分别为 74.8% 和 61.7%。生物反应器中微生物分类学组成的研究表明Methanosarcina 和 Methanosaeta是最普遍的产甲烷菌,而DesulfotomaculumSyntrophobacter属是主要的 SRB。在鉴定的 SRB 中,Desulfotomaculum intricatum(99% 同一性)和Desulfotomaculum acetoxidans(96%)是能够使用乙酸盐的最丰富的细菌序列。

更新日期:2021-08-20
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