To explore the differently expressed cytokines and chemokines to understand the pathways that lead to herpes simplex encephalitis (HSE). Mice in the experimental group were inoculated intracranially with HSV-1. A high-throughput cytokine chip assay was employed to assess the expression of cytokines/chemokines in the mice brain. GO, KEGG, and PPIs analyses were used to investigate the biological process (BP), pathways and interaction network of the differently expressed proteins (DEPs) in HSE. 13 DEPs and various proteins-related signal pathways were identified in HSE, including three new factors (IL-1α, MIP-1γ, and sTNF RI). The proteins were mainly implicated in leukocyte activation and chemotaxis. Additionally, the DEPs constituted a pivotal protein interaction network where IL-6 might be a mediator. 13 DEPs and a series of related signal pathways were associated with the pathophysiological mechanisms responsible for HSE. IL-6 might be a key mediator in the inflammatory responses to the disease.

探索不同表达的细胞因子和趋化因子，以了解导致单纯疱疹脑炎 (HSE) 的途径。实验组小鼠颅内接种HSV-1。采用高通量细胞因子芯片测定来评估小鼠大脑中细胞因子/趋化因子的表达。GO、KEGG和PPIs分析用于研究HSE中不同表达蛋白（DEPs）的生物学过程（BP）、通路和相互作用网络。在 HSE 中鉴定出 13 个 DEP 和各种蛋白质相关信号通路，包括三个新因子（IL-1α、MIP-1γ 和 sTNF RI）。这些蛋白质主要与白细胞活化和趋化性有关。此外，DEP 构成了一个关键的蛋白质相互作用网络，其中 IL-6 可能是一种介质。13 个 DEP 和一系列相关信号通路与 HSE 的病理生理机制相关。IL-6 可能是对疾病的炎症反应的关键介质。