当前位置: X-MOL 学术Genomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana
Genomics ( IF 4.4 ) Pub Date : 2021-08-12 , DOI: 10.1016/j.ygeno.2021.08.006
Saurabh Chaudhary 1 , Ibtissam Jabre 2 , Naeem H Syed 3
Affiliation  

How stable and temperature-dependent variations in DNA methylation and nucleosome occupancy influence alternative splicing (AS) remains poorly understood in plants. To answer this, we generated transcriptome, whole-genome bisulfite, and MNase sequencing data for an epigenetic Recombinant Inbred Line (epiRIL) of A. thaliana at normal and cold temperature. For comparative analysis, the same data sets for the parental ecotype Columbia (Col-0) were also generated, whereas for DNA methylation, previously published high confidence methylation profiles of Col-0 were used. Significant epigenetic differences in an identical genetic background were observed between Col-0 and epiRIL lines under normal and cold temperatures. Our transcriptome data revealed that differential DNA methylation and nucleosome occupancy modulate expression levels of many genes and AS in response to cold. Collectively, DNA methylation and nucleosome levels exhibit characteristic patterns around intron-exon boundaries at normal and cold conditions, and any perturbation in them, in an identical genetic background is sufficient to modulate AS in Arabidopsis.



中文翻译:

相同遗传背景中的表观遗传差异调节拟南芥的可变剪接

DNA甲基化和核小体占据的稳定和温度依赖性变化如何影响植物中的选择性剪接(AS)仍然知之甚少。为了回答这个问题,我们生成了拟南芥表观遗传重组近交系 (epiRIL) 的转录组、全基因组亚硫酸氢盐和 MNase 测序数据在正常和低温下。对于比较分析,还生成了与亲本生态型 Columbia (Col-0) 相同的数据集,而对于 DNA 甲基化,使用了先前发表的 Col-0 的高置信甲基化谱。在正常和低温下,在 Col-0 和 EpiRIL 系之间观察到相同遗传背景下的显着表观遗传差异。我们的转录组数据显示,差异 DNA 甲基化和核小体占据调节许多基因和 AS 的表达水平以响应寒冷。总的来说,DNA 甲基化和核小体水平在正常和寒冷条件下在内含子-外显子边界周围表现出特征性模式,并且在相同的遗传背景下,它们中的任何扰动都足以调节拟南芥中的 AS。

更新日期:2021-08-24
down
wechat
bug