Bacterial growth and degradation experiments were conducted on carbazole (CBZ), fluorene (FLU) and dibenzothiophene (DBT) individually and in various mixture combinations using an efficient polynuclear aromatic hydrocarbon (PAH) degrading bacterial strain, Pseudomonas aeruginosa RS1. In single component systems, bacterial growth on CBZ (specific growth rate, μ = 0.99 day⁻¹) was much higher compared to that on FLU (μ = 0.38 day⁻¹) and DBT (μ = 0.33 day⁻¹) and bacterial growth was inhibited in the presence of FLU and DBT in binary (μ = 0.64 day⁻¹) and ternary (μ = 0.75 day⁻¹) mixtures. Multisubstrate additive modelling indicated growth inhibition in all the systems. The degradation of the compounds was significantly inhibited in binary mixtures. While the degradation of the compounds in binary mixtures varied from 35 ± 4% to 73 ± 3%, their degradation varied from 61 ± 5% to 91 ± 4%, when applied as sole substrates and from 77 ± 3% to 96 ± 3%, when applied in a ternary mixture. Degradation experiments were also conducted in ternary mixtures using a 2³ full factorial design and the results were examined using analysis of variance (ANOVA) and Tukey's honest significant difference (HSD) tests. At a low concentration of the heterocyclics, CBZ and DBT (5 mg L⁻¹ each), the degradation of the PAH, FLU, was significantly enhanced (from 81 ± 1% to 93 ± 0.3%) when its concentration was increased from 5 to 30 mg L⁻¹. The full factorial design can provide valuable insights into substrate interaction effects in mixtures.

中文翻译：

---

含三环同环和杂环多环芳烃的多组分体系中底物相互作用效应的阐明

使用一种高效的多环芳烃 (PAH) 降解细菌菌株铜绿假单胞菌RS1 ，分别对咔唑 (CBZ)、芴 (FLU) 和二苯并噻吩 (DBT) 进行细菌生长和降解实验，并以各种混合物组合进行。在单组分系统中，CBZ 上的细菌生长（比生长率，μ = 0.99 天^-1）远高于 FLU（μ = 0.38 天^-1）和 DBT（μ = 0.33 天^-1）上的细菌生长和细菌生长在二元（μ = 0.64 天^-1）和三元（μ = 0.75 天）存在 FLU 和 DBT 的情况下，^-1 ) 混合物。多底物加成模型表明所有系统中的生长抑制。二元混合物中化合物的降解受到显着抑制。虽然二元混合物中化合物的降解范围从 35 ± 4% 到 73 ± 3% 不等，但当用作唯一底物时，它们的降解范围从 61 ± 5% 到 91 ± 4% 和从 77 ± 3% 到 96 ± 3 %，当以三元混合物应用时。还使用 2 ³全因子设计在三元混合物中进行降解实验，并使用方差分析 (ANOVA) 和 Tukey 诚实显着差异 (HSD) 检验检查结果。在低浓度的杂环、CBZ 和 DBT (5 mg L ^-1每个），当其浓度从 5 增加到 30 mg L ^-1时，PAH、FLU 的降解显着增强（从 81 ± 1% 到 93 ± 0.3%）。全因子设计可以提供对混合物中底物相互作用效应的宝贵见解。