Long non-coding RNAs (lncRNAs) exert vital functions in the occurrence and development of various tumours. The aim of this study was to examine the regulatory effect and underlying molecular mechanism of lncRNA small nucleolar RNA host gene 14 (SNHG14) on the proliferation, invasion and migration of thyroid tumour cells. The expression of SNHG14 in thyroid tumour cell lines was determined using qRT-PCR. CCK-8 and western blot were used to detect the effects of SNHG14 on proliferation and apoptosis of thyroid tumour cells. The effect of SNHG14 on the migration and invasion of thyroid tumour cells was analyzed using immunofluorescence, wound-healing and transwell assays. A targeting relationship between SNHG14 and miR-93-5p was determined using bioinformatics software and luciferase reporter assays. In addition, CCK-8, immunofluorescence, wound-healing and transwell assays were applied to demonstrate that SNHG14 promoted the proliferation, migration and invasion of thyroid tumour cells by targeting miR-93-5p. The biological function of SNHG14 in vivo was explored through a xenograft model and immunohistochemistry. SNHG14 was upregulated in thyroid tumour cells compared with normal cells. Downregulation of SNHG14 effectively reduced the proliferation, migration and invasion of TPC-1 cells, and induced cell apoptosis. Moreover, SNHG14 directly targeted miR-93-5p and there was a negative correlation between them. Further functional experiments illustrated that miR-93-5p overexpression dramatically reversed the promoting role of SNHG14 in proliferation, migration and invasion of TPC-1 cells. Our results demonstrated that SNHG14 promotes the proliferation, invasion and migration of thyroid tumour cells by downregulating miR-93-5p.

长链非编码 RNA (lncRNA) 在各种肿瘤的发生和发展中发挥重要作用。本研究旨在探讨lncRNA小核仁RNA宿主基因14（SNHG14）对甲状腺肿瘤细胞增殖、侵袭和迁移的调控作用及其潜在分子机制。使用 qRT-PCR 测定 SNHG14 在甲状腺肿瘤细胞系中的表达。采用CCK-8和western blot检测SNHG14对甲状腺肿瘤细胞增殖和凋亡的影响。使用免疫荧光、伤口愈合和 transwell 测定分析 SNHG14 对甲状腺肿瘤细胞迁移和侵袭的影响。使用生物信息学软件和荧光素酶报告基因分析确定 SNHG14 和 miR-93-5p 之间的靶向关系。此外，CCK-8、免疫荧光、应用伤口愈合和 transwell 试验证明 SNHG14 通过靶向 miR-93-5p 促进甲状腺肿瘤细胞的增殖、迁移和侵袭。SNHG14的生物学功能通过异种移植模型和免疫组织化学对体内进行了探索。与正常细胞相比，SNHG14 在甲状腺肿瘤细胞中上调。下调 SNHG14 可有效降低 TPC-1 细胞的增殖、迁移和侵袭能力，并诱导细胞凋亡。此外，SNHG14直接靶向miR-93-5p，二者呈负相关。进一步的功能实验表明，miR-93-5p 过表达显着逆转了 SNHG14 在 TPC-1 细胞增殖、迁移和侵袭中的促进作用。我们的研究结果表明，SNHG14 通过下调 miR-93-5p 促进甲状腺肿瘤细胞的增殖、侵袭和迁移。