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Identification of major consensus and epistatic QTLs for late leaf spot resistance in a cultivated groundnut breeding line VG 9514
Euphytica ( IF 1.9 ) Pub Date : 2021-08-11 , DOI: 10.1007/s10681-021-02908-7
Suvendu Mondal 1, 2 , Anand M. Badigannavar 1, 2 , Babu N. Motagi 3
Affiliation  

A late leaf spot resistant breeding line VG 9514 was bred through interspecific hybridization between Arachis hypogaea L. and Arachis cardenasii. Genetic study involving segregation for late leaf spot resistance in 164 F2 and recombinant inbred line populations derived from a cross between VG 9514 X TAG 24 revealed duplicate recessive resistance for the disease. Integration of newly developed SSR markers from A02 chromosome with existing linkage map generated a new genetic linkage map with 278 markers and 2679.1 cM map distances. QTL mapping involving this genetic linkage map and phenotypic field score of late leaf spot identified two major consensus additive QTLs in the A02 chromosome of cultivated groundnut. Epistatic interaction between these two major QTLs was also noticed through an epistatic QTL analysis in Ici-Mapping 4.1. In all the fourteen epistatic QTLs, a common component locus was remained within the major additive QTL at 90 cM in chromosome A02. Sequence analysis within the major additive QTL peaks revealed open reading frame of genes that code LRR domain containing proteins which are involved in disease resistance of crop plants.



中文翻译:

栽培花生育种系 VG 9514 晚叶斑病抗性的主要共有 QTL 和上位 QTL 的鉴定

花生花生进行种间杂交选育出晚叶斑病抗性育种品系VG 9514 。164 F 2晚叶斑病抗性分离遗传研究来自 VG 9514 X TAG 24 杂交的重组自交系群体显示出对该疾病的双重隐性抗性。来自 A02 染色体的新开发的 SSR 标记与现有连锁图的整合生成了具有 278 个标记和 2679.1 cM 图距离的新遗传连锁图。涉及该遗传连锁图的 QTL 定位和晚叶斑病的表型场评分在栽培花生的 A02 染色体中确定了两个主要的共有附加 QTL。通过 Ici-Mapping 4.1 中的上位 QTL 分析也注意到了这两个主要 QTL 之间的上位相互作用。在所有 14 个上位 QTL 中,A02 染色体上 90 cM 处的主要加性 QTL 中保留了一个共同的组成基因座。

更新日期:2021-08-12
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