ABSTRACT

Objectives

Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications.

Methods

We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts.

Results

The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications.

Discussion

Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.

中文翻译：

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基于硫醇的氧化还原系统可以用作合成生物学应用的一部分吗？

摘要

目标

合成生物学源于分子生物学和工程方法，旨在开发新颖的、受生物启发的系统，用于从生物计算到药物生产的工业和基础研究应用。令人惊讶的是，氧化还原蛋白（硫氧还蛋白、谷氧还蛋白、过氧化氧还蛋白）和其他基于硫醇的氧化还原系统尚未广泛应用于许多这些合成生物学应用中。

方法

我们回顾了基于硫醇的氧化还原系统以及使用硫醇依赖性部件的合成生物学应用的开发。

结果

使用硫醇基部件的应用包括促进细胞质二硫键键合的电路的开发、生物计算和肠道疾病的治疗。我们认为，基因编码的氧化还原传感器、硫醇基生物材料和细胞内过氧化氢发生器也可能是合成生物学应用的有价值的组件。

讨论

基于硫醇的系统在细胞氧化还原代谢、抗氧化防御和信号传导中发挥多种作用，因此可以为合成生物学应用提供广泛且多样化的组件、零件和设备组合。然而，限制氧化还原系统在合成生物学应用中采用的因素包括硫醇基组分的正交性、硫醇基系统表征方法的限制以及对这些系统设计原理的不完全理解。