A simple, selective and sensitive “signal-on” electrogenerated chemiluminescence (ECL) biosensing method was developed for matrix metalloproteinase 2 (MMP-2). Ru(bpy)₃²⁺, gold nanoparticles (AuNPs) and Nafion were modified onto glassy carbon electrode (GCE) to form Ru(bpy)₃²⁺/AuNPs/Nafion/GCE as sensitive ECL platform and then ferrocene (Fc) labeled peptide was assembled onto the modified electrode to form ECL biosensing platform. The ECL intensity increased when the ECL biosensing electrode reacted with MMP-2 because of MMP-2-induced cleavage of Fc labeled peptide. The ECL method was applied to determine MMP-2 with detection limit of 0.3 ng/mL and one-step recognition, which is promising for point-of-care test of protease.

中文翻译：

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用于测定基质金属蛋白酶 2 的“Signal-on”电生成化学发光生物传感方法

为基质金属蛋白酶 2 (MMP-2) 开发了一种简单、选择性和灵敏的“信号开启”电生成化学发光 (ECL) 生物传感方法。将Ru(bpy) ₃ ²⁺、金纳米粒子(AuNPs)和Nafion修饰到玻碳电极(GCE)上形成Ru(bpy) ₃ ²⁺ /AuNPs/Nafion/GCE作为敏感的ECL平台，然后用二茂铁(Fc)标记肽组装到修饰电极上形成ECL生物传感平台。当 ECL 生物传感电极与 MMP-2 反应时，由于 MMP-2 诱导 Fc 标记肽的切割，ECL 强度增加。采用ECL法测定MMP-2，检出限为0.3 ng/mL，一步识别，有望用于蛋白酶的床旁检测。