Abstract

HCV patients are usually under substantial oxidative stress because of viral infection. A total of 177 patients with HCV infection and 198 age- and sex-matched healthy controls were enrolled in this study. We evaluated the urinary levels of 8-oxo-7, 8-dihydro-2’deoxyguanosine (8-oxodGuo) and 8-oxo-7, 8-dihydroguanosine (8-oxoGuo) in patients with HCV infection and explored the factors affecting the urinary 8-oxodGuo or 8-oxoGuo levels. Biomarkers of liver function, cancer, and inflammation were determined. Nonparametric correlations were used to evaluate the correlation between 8-oxoGuo or 8-oxodGuo and various laboratory biochemical indicators. Results showed that the levels of urinary 8-oxoGuo both in male and female patients with HCV infection were significantly higher than those in healthy controls (both p < 0.0001), while the urinary 8-oxodGuo levels only in male patients with HCV infection were significantly higher than those in healthy controls (p < 0.01). Urinary 8-oxoGuo was significantly associated with the white blood cell count, C-reactive protein level, and 8-oxodGuo level (p = 0.016, p = 0.003, and p = 0.000, respectively). Urinary 8-oxodGuo was significantly associated with the white blood cell count and 8-oxoGuo level (p = 0.018 and p = 0.000, respectively). A regression equation of urinary 8-oxoGuo or 8-oxodGuo was also established using the biomarkers in plasma. The results suggested that patients with a high C-reactive protein level are likely to have high urinary 8-oxoGuo levels as well, which may be useful for assessing the level of inflammation and oxidative stress in HCV patients.

Supplemental data for this article is available online at https://doi.org/10.1080/15257770.2021.1961272 .

摘要

由于病毒感染，HCV 患者通常处于大量氧化应激之下。本研究共招募了 177 名 HCV 感染患者和 198 名年龄和性别匹配的健康对照。我们评估了 HCV 感染患者尿液中 8-oxo-7, 8-dihydro-2'deoxyguanosine (8-oxodGuo) 和 8-oxo-7, 8-dihydroguanosine (8-oxoGuo) 的水平，并探讨了影响尿 8-oxodGuo 或 8-oxoGuo 水平。确定了肝功能、癌症和炎症的生物标志物。非参数相关性用于评估 8-oxoGuo 或 8-oxodGuo 与各种实验室生化指标之间的相关性。结果显示，男性和女性HCV感染患者的尿8-oxoGuo水平均显着高于健康对照组（均p < 0.0001），而男性HCV感染患者的尿8-oxodGuo水平显着高于健康对照组（p < 0.01）。尿液 8-oxoGuo 与白细胞计数、C 反应蛋白水平和 8-oxodGuo 水平显着相关（分别为 p = 0.016、p = 0.003 和 p = 0.000）。尿液 8-oxoGuo 与白细胞计数和 8-oxoGuo 水平显着相关（分别为 p = 0.018 和 p = 0.000）。还利用血浆中的生物标志物建立了尿 8-oxoGuo 或 8-oxodGuo 的回归方程。结果表明，具有高 C 反应蛋白水平的患者可能也具有高尿 8-oxoGuo 水平，这可能有助于评估 HCV 患者的炎症和氧化应激水平。

本文的补充数据可在 https://doi.org/10.1080/15257770.2021.1961272 在线获得。