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Hemoglobin quantification in red blood cells via dry mass mapping based on UV absorption
Journal of Biomedical Optics ( IF 3.5 ) Pub Date : 2021-08-01 , DOI: 10.1117/1.jbo.26.8.086501
Nischita Kaza 1 , Ashkan Ojaghi 2 , Francisco Robles 2
Affiliation  

Significance: The morphological properties and hemoglobin (Hb) content of red blood cells (RBCs) are essential biomarkers to diagnose or monitor various types of hematological disorders. Label-free mass mapping approaches enable accurate Hb quantification from individual cells, serving as promising alternatives to conventional hematology analyzers. Deep ultraviolet (UV) microscopy is one such technique that allows high-resolution, molecular imaging, and absorption-based mass mapping. Aim: To compare UV absorption-based mass mapping at four UV wavelengths and understand variations across wavelengths and any assumptions necessary for accurate Hb quantification. Approach: Whole blood smears are imaged with a multispectral UV microscopy system, and the RBCs’ dry masses are computed. This approach is compared to quantitative phase imaging-based mass mapping using data from an interferometric UV imaging system. Results: Consistent Hb mass and mean corpuscular Hb values are obtained at all wavelengths, with the precision of the single-cell mass measurements being nearly identical at 220, 260, and 280 nm but slightly lower at 300 nm. Conclusions: A full hematological analysis (including white blood cell identification and characterization, and Hb quantification) may be achieved using a single UV illumination wavelength, thereby improving the speed and cost-effectiveness.

中文翻译:

通过基于紫外线吸收的干质量映射定量红细胞中的血红蛋白

意义:红细胞 (RBC) 的形态学特性和血红蛋白 (Hb) 含量是诊断或监测各种类型血液病的重要生物标志物。无标记质量映射方法可以对单个细胞进行准确的 Hb 定量,作为传统血液分析仪的有前途的替代品。深紫外 (UV) 显微镜就是这样一种技术,它允许高分辨率、分子成像和基于吸收的质量映射。目的:比较四个 UV 波长下基于 UV 吸收的质量映射,并了解不同波长的变化以及准确 Hb 定量所需的任何假设。方法:使用多光谱紫外显微镜系统对全血涂片进行成像,并计算红细胞的干质量。将此方法与使用来自干涉测量 UV 成像系统的数据的基于定量相位成像的质量映射进行比较。结果:在所有波长下均获得一致的 Hb 质量和平均红细胞 Hb 值,单细胞质量测量的精度在 220、260 和 280 nm 处几乎相同,但在 300 nm 处略低。结论:可以使用单个紫外线照射波长实现完整的血液学分析(包括白细胞鉴定和表征以及 Hb 定量),从而提高速度和成本效益。和 280 nm,但在 300 nm 处略低。结论:可以使用单个紫外线照射波长实现完整的血液学分析(包括白细胞鉴定和表征以及 Hb 定量),从而提高速度和成本效益。和 280 nm,但在 300 nm 处略低。结论:可以使用单个紫外线照射波长实现完整的血液学分析(包括白细胞鉴定和表征以及 Hb 定量),从而提高速度和成本效益。
更新日期:2021-08-11
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