Contrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy deconvolution. We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast.

中文翻译：

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通过低对比度噪声图像堆栈导出高对比度荧光显微镜图像

荧光显微镜图像中的对比度允许通过强度差异来区分不同结构。然而，点扩散函数和噪声等因素可能会降低它，影响其可解释性。我们发现，与平均和 Richardson-Lucy 反卷积相比，可以利用一堆图像中发射器的波动来实现更高的对比度。我们在包括组织在内的四个越来越具有挑战性的样本上测试了我们的方法，在这种情况下，结果与结构照明显微镜在对比度方面获得的结果相当。