Objectives. This study investigated the functional mechanism of transmembrane protein 100 (TMEM100) as a tumor inhibitor gene in CRC cells and offered a reference for the treatment of CRC. Methods. The mRNA expression data of CRC were acquired from the TCGA database to mine differentially expressed mRNAs. The role of TMEM100 in the progression of CRC cells was evaluated by MTT, colony formation, scratch healing, and Transwell assays. The influence of TMEM100 on the TGF-β signaling pathway was detected by western blot. Results. TMEM100 was markedly lowly expressed in CRC. CRC cell growth was significantly suppressed by overexpressing TMEM100 but noticeably facilitated by silencing TMEM100. Overexpression of TMEM100 inhibited the activation of the TGF-β signaling pathway, thus inhibiting malignant progression of CRC. Conclusion. TMEM100 is lowly expressed in CRC, which can suppress CRC cell growth by regulating the TGF-β signaling pathway.

中文翻译：

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TMEM100 调节 TGF-β 信号通路以抑制结直肠癌进展

目标。本研究探讨跨膜蛋白100（TMEM100）作为肿瘤抑制基因在结直肠癌细胞中的作用机制，为结直肠癌的治疗提供参考。方法。从TCGA数据库获取CRC的mRNA表达数据，以挖掘差异表达的mRNA。TMEM100 在 CRC 细胞进展中的作用通过 MTT、集落形成、划痕愈合和 Transwell 测定进行评估。Western blot检测TMEM100对TGF- β信号通路的影响。结果. TMEM100 在 CRC 中显着低表达。过表达 TMEM100 显着抑制 CRC 细胞生长，但沉默 TMEM100 显着促进了 CRC 细胞生长。TMEM100的过表达抑制了TGF- β信号通路的激活，从而抑制了CRC的恶性进展。结论。TMEM100在CRC中低表达，可通过调节TGF- β信号通路抑制CRC细胞生长。