Herein, a surface plasmon resonance (SPR)-based nanosensor was fabricated using molecular imprinting technique for the selective and sensitive detection of amitrole, a commonly used highly toxic pesticide. For this purpose, polymeric nanofilms involving N-methacryloyl-l-tryptophan methyl ester and ethylene glycol dimethacrylate which are functional monomer and cross-linker, respectively, were prepared on the SPR chips surface via the ultravoilet polymerization method. The nanofilms were characterized by several techniques such as ellipsometry, Fourier transform infrared-attenuated total reflection, atomic force microscope and contact angle measurements. The kinetic analyses for amitrole detection were examined through amitrole-imprinted and non-imprinted SPR sensor chips. The developed imprinted sensor showed high selectivity to the amitrole molecule than similar molecules and a good linear relationship for the 0.06-11.90 nM concentration range with a low limit of detection value of 0.037 nM. The high imprinting efficiency (I.F: 62.38) of the amitrole-imprinted SPR sensor was determined by comparing it with the non-imprinted SPR sensor. The most suitable model of this sensor is the Langmuir adsorption model. To statistically assess the reusability of the sensor, intraday experiments were tested three times with five replicates. The relative standard deviation% value less than <1.5 indicates high reproducibility for both sensor production and reproducibility of the method. The prepared sensor was also applied efficiently for the selective detection of the amitrole in spiked samples prepared from vegetables to evaluate the matrix effect. As a result, amitrole-imprinted SPR sensors have been estimated to be highly selective, fast responsive, easy to use, reusable and sensitive in detecting amitrole in both natural source and aqueous solutions. Label-free amitrole detection was performed by an aminoacid-based SPR sensor fabricated without the need for complex coupling processes.

中文翻译：

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基于分子印迹纳米传感器的阿米特罗灵敏选择性检测

在此，使用分子印迹技术制造了一种基于表面等离子共振 (SPR) 的纳米传感器，用于选择性和灵敏地检测常用的剧毒农药阿米特罗。为此，涉及 N-甲基丙烯酰基的聚合物纳米膜通过紫外聚合法在SPR芯片表面制备了分别作为功能单体和交联剂的-色氨酸甲酯和乙二醇二甲基丙烯酸酯。纳米薄膜通过多种技术进行表征，例如椭圆光度法、傅里叶变换红外衰减全反射、原子力显微镜和接触角测量。通过阿米特罗印记和非印记 SPR 传感器芯片检查阿米特罗检测的动力学分析。开发的印迹传感器对阿米托罗分子的选择性高于同类分子，在0.06-11.90 nM浓度范围内具有良好的线性关系，检测下限为0.037 nM。高压印效率（IF：62. 38) 通过将其与非印迹 SPR 传感器进行比较来确定 amitrol 印迹 SPR 传感器。该传感器最适合的模型是朗缪尔吸附模型。为了从统计上评估传感器的可重复使用性，日内实验进行了 3 次测试，重复 5 次。小于 <1.5 的相对标准偏差% 值表明传感器生产和方法的再现性都具有高再现性。制备的传感器还有效地用于选择性检测从蔬菜制备的加标样品中的阿米特罗，以评估基质效应。因此，阿米特罗印记的 SPR 传感器被认为在检测天然来源和水溶液中的阿米特罗时具有高度选择性、快速响应、易于使用、可重复使用和灵敏的特点。