Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging method that has the potential to transform the field of metabolomics. This is in part due to LAESI-MS being an ambient ionization method that requires minimal sample preparation and uses (endogenous) water for in situ analysis. This application note details the employment of the "LAESI microscope" source to perform spatially resolved MS analysis of cells and MS imaging (MSI) of tissues at high spatial resolution. This source configuration utilizes a long-working-distance reflective objective that permits both visualization of the sample and a smaller LAESI laser beam profile than conventional LAESI setups. Here, we analyzed 200 single cells of Allium cepa (red onion) and imaged Fittonia argyroneura (nerve plant) in high spatial resolution using this source coupled to a Fourier transform mass spectrometer for high-mass-resolution and high-mass-accuracy metabolomics.

中文翻译：

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使用具有更高空间分辨率的激光消融电喷雾电离质谱法进行环境单细胞分析和天然组织成像。

激光烧蚀电喷雾电离质谱 (LAESI-MS) 是一种新兴方法，具有改变代谢组学领域的潜力。这部分是因为 LAESI-MS 是一种环境电离方法，需要最少的样品制备并使用（内源性）水进行原位分析。本应用说明详细介绍了使用“LAESI 显微镜”源以高空间分辨率对细胞进行空间分辨 MS 分析和对组织进行 MS 成像 (MSI)。这种源配置采用长工作距离反射物镜，与传统的 LAESI 设置相比，它允许样品的可视化和更小的 LAESI 激光束轮廓。这里，