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Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris.
Biotechniques ( IF 2.7 ) Pub Date : 2021-08-10 , DOI: 10.2144/btn-2021-0039
Junhao Cheng 1 , Mingkun Wu 1 , Ren Zhong 1 , Dayong Si 1 , Geng Meng 1 , Rijun Zhang 1 , Yueping Zhang 1
Affiliation  

The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficient and cost-effective assembly of multiple exons of interest genes into different expression plasmids in both Escherichia coli and Pichia pastoris. The high cloning efficiency and low cost of the system make it ideal for a novel workflow for the assembly of intron-bearing genes for expression in two different expression hosts.

中文翻译:

来自基因组 DNA 的外显子的快速金门组装,用于在大肠杆菌和毕赤酵母中表达蛋白质。

介绍了用于生成多外显子基因表达构建体的快速、单步克隆系统的开发。该系统允许将多个感兴趣基因的外显子高效且经济地组装到大肠杆菌毕赤酵母中的不同表达质粒中。该系统的高克隆效率和低成本使其成为组装内含子基因以在两种不同表达宿主中表达的新型工作流程的理想选择。
更新日期:2021-08-10
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