Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.

中文翻译：

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一种快速、无损检测疾病相关病灶和受伤叶片的方法。

台盼蓝染色是可视化植物叶片病害和伤口反应的经典方法，但它涉及使用有毒化学物质且耗时（2-3 天）。在这里，研究人员使用标准实验室设备建立了近红外扫描，作为与台盼蓝染色相比，分析叶片损伤的一种快速且无损的方法。来自马铃薯、番茄、菠菜、草莓和拟南芥植物的病原体接种和受伤的叶子被用于概念验证。结果表明，这种新开发的近红外扫描方案给出了与台盼蓝染色相同的结果。此外，在斐济制作了一个宏来量化叶片损伤。新方案省时、无损、不含化学物质，可用于高通量研究。