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Dietary tryptophan supplementation does not affect growth but increases brain serotonin level and modulates the expression of some liver genes in zebrafish (Danio rerio)
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2021-08-09 , DOI: 10.1007/s10695-021-00994-x
Cláudia Teixeira 1, 2 , Pedro Rodrigues 1, 2 , Paula Serrão 3 , Luís Figueira 3 , Laura Guimarães 2 , Luís Oliva Teles 1, 2 , Helena Peres 1, 2 , António Paulo Carvalho 1, 2
Affiliation  

This study aimed at assessing the effects of the dietary tryptophan (Trp) supplementation on growth and feed utilization, brain serotonin content, and expression of selected liver genes (involved in the liver serotonin pathway, protein synthesis degradation, and antioxidant activity) in zebrafish. A growth trial was conducted with zebrafish juveniles fed five experimental isoproteic (40%DM) and isolipidic (8%DM) fishmeal-based diets containing graded levels of Trp: a Trp-non-supplemented diet (diet Trp0, with 0.22% Trp) and four Trp-supplemented diets containing 2–16 times higher Trp content (diets Trp2, Trp4, Trp8, and Trp16 with 0.40, 0.91, 2.02, and 3.34% Trp, respectively). Diets were tested in quadruplicate, with fish being fed twice a day, 6 days a week for 6 weeks to apparent visual satiation. At the end of the trial, growth performance and feed utilization were assessed, and fish from all experimental groups were sampled for whole-body composition analysis. In addition, fish fed low (Trp0), medium (Trp4), and high (Trp16) Trp diets were also sampled for analysis of brain serotonin content and liver gene expression. Tested tryptophan levels did not influence growth performance nor feed intake. However, values of energy and nitrogen retention as well as body energy content indicate a better feed utilization with diets containing around 0.9% and 2.0% DM Trp. Brain serotonin content increased with increasing dietary tryptophan levels. In addition, regarding liver genes, dietary treatment had a modulatory effect on the expression of Htr1aa and Htr2cl1 genes (encoding for serotonin receptors), TPH1a gene (encoding for tryptophan hydroxylase, the rate-limiting enzyme in the synthesis of serotonin from tryptophan), TOR gene (involved in protein synthesis), and Keap1 gene (involved in antioxidant responses).



中文翻译:

膳食色氨酸补充剂不会影响生长,但会增加脑血清素水平并调节斑马鱼中某些肝脏基因的表达 (Danio rerio)

本研究旨在评估膳食色氨酸 (Trp) 补充剂对斑马鱼生长和饲料利用、脑血清素含量和选定肝脏基因(参与肝脏血清素途径、蛋白质合成降解和抗氧化活性)表达的影响。对斑马鱼幼鱼进行了生长试验,其中饲喂五种实验性等蛋白 (40% DM) 和等脂 (8% DM) 鱼粉,其中含有分级的 Trp 水平:不添加 Trp 的饮食(饮食 Trp0,含 0.22% Trp)和四种 Trp 补充饮食,其 Trp 含量高出 2-16 倍(Trp2、Trp4、Trp8 和 Trp16 的 Trp 含量分别为 0.40、0.91、2.02 和 3.34%)。饮食测试一式四份,每天喂鱼两次,每周 6 天,持续 6 周,以达到明显的视觉饱腹感。在审判结束时,评估了生长性能和饲料利用率,并对所有实验组的鱼进行了全身成分分析。此外,还对喂食低 (Trp0)、中 (Trp4) 和高 (Trp16) Trp 饮食的鱼进行取样,用于分析脑血清素含量和肝脏基因表达。测试的色氨酸水平不影响生长性能和采食量。然而,能量和氮保留值以及身体能量含量表明日粮中含有约 0.9% 和 2.0% DM Trp 的饲料利用率更高。脑血清素含量随着膳食色氨酸水平的增加而增加。此外,关于肝脏基因,饮食处理对 Htr1aa 和 Htr2cl1 基因(编码血清素受体)、TPH1a 基因(编码色氨酸羟化酶、

更新日期:2021-08-10
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