Among the diverse nanomaterials, gold nanoparticles (AuNps) are utilised for various therapeutic application due to the distinct physical, chemical properties and biocompatibility. Synthesis of gold nanoparticles using plants is the promising route. This method is low cost, eco-friendly and higher biological activities. In this present study, Gold nanoparticles were synthesised from fruit extract of Terminalia bellirica fruit extract. Their anticancer and anti-inflammatory activity was evaluated against colorectal cancer cell line (HT29) and TNBS-induced zebrafish model. Highly stable tannin capped gold nanoparticles were synthesised from fruit extract broth of Terminalia bellirica rapidly. Structural and functional properties of the synthesised nanoparticles were studied by Fourier transform infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM) equipped with energy-dispersive atomic X-ray spectroscopy (EDAX) and X-ray diffraction (XRD). All the characterisation studies reveal highly stable, crystalline, phytochemicals, mainly tannin doped, spherical, 28 nm controlled sized gold nanoparticles. The molecular mechanism of anticancer activity was studied by determining cancer markers’ expression, which was studied using quantitative real-time polymerase chain reaction (qPCR). Antioxidative enzymes’ status and apoptosis changes were also investigated. Synthesised nanoparticles brought a drastic reduction of all the tested cancer markers’ expression. Notable changes in antioxidative enzymes’ status and a good sign of apoptosis were observed in nanoparticles’ treatment. The anti-inflammatory activity was studied against TNBS-induced zebrafish model, which was confirmed by determining inflammatory markers’ expression TNF-α, iNOS (induced Nitric Oxide Synthase) and histopathological examination. Nanoparticles’ treatment recorded a drastic reduction of inflammatory markers’ expression. No marked sign of inflammation was also observed in histopathological analysis of the nanoparticles’ treatment group. The present study suggests the possible utilisation of T. bellirica-mediated gold nanoparticles as an effective therapeutic agent against a prolonged inflammatory disease that progressively develops into cancer.

在多种纳米材料中，金纳米粒子 (AuNps) 因其独特的物理、化学性质和生物相容性而被用于各种治疗应用。利用植物合成金纳米粒子是有前途的途径。该方法成本低、环境友好、生物活性较高。在本研究中，金纳米粒子是从榄仁果实提取物的果实提取物中合成的。针对结直肠癌细胞系 (HT29) 和 TNBS 诱导的斑马鱼模型评估了它们的抗癌和抗炎活性。以榄仁果提取液为原料合成高稳定性单宁包覆金纳米粒子迅速。通过傅里叶变换红外光谱 (FTIR)、配备能量色散原子 X 射线光谱 (EDAX) 和 X 射线衍射 (XRD) 的场发射扫描电子显微镜 (FESEM) 研究了合成纳米颗粒的结构和功能特性。所有表征研究都揭示了高度稳定的结晶植物化学物质，主要是单宁掺杂的球形、28 nm 可控尺寸的金纳米颗粒。通过确定癌症标志物的表达来研究抗癌活性的分子机制，使用定量实时聚合酶链反应（qPCR）进行研究。还研究了抗氧化酶的状态和细胞凋亡的变化。合成的纳米颗粒大大降低了所有测试癌症标志物的表达。在纳米粒子的处理中观察到抗氧化酶状态的显着变化和细胞凋亡的良好迹象。针对 TNBS 诱导的斑马鱼模型研究了抗炎活性，通过确定炎症标志物的表达 TNF-α、iNOS（诱导一氧化氮合酶）和组织病理学检查证实了这一点。纳米粒子的治疗记录了炎症标志物表达的急剧减少。在纳米粒子治疗组的组织病理学分析中也没有观察到明显的炎症迹象。本研究表明可能利用 iNOS（诱导一氧化氮合酶）和组织病理学检查。纳米粒子的治疗记录了炎症标志物表达的急剧减少。在纳米粒子治疗组的组织病理学分析中也没有观察到明显的炎症迹象。本研究表明可能利用 iNOS（诱导一氧化氮合酶）和组织病理学检查。纳米粒子的治疗记录了炎症标志物表达的急剧减少。在纳米粒子治疗组的组织病理学分析中也没有观察到明显的炎症迹象。本研究表明可能利用T. bellirica介导的金纳米粒子作为一种有效的治疗剂，可对抗逐渐发展为癌症的长期炎性疾病。