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Nephroprotective and anti-inflammatory potential of aqueous extract from Persea americana seeds against cadmium-induced nephrotoxicity in Wistar rats
Biometals ( IF 3.5 ) Pub Date : 2021-08-07 , DOI: 10.1007/s10534-021-00333-w
Olukemi Adetutu Osukoya 1 , Babatunji Emmanuel Oyinloye 1, 2, 3 , Basiru Olaitan Ajiboye 1, 3, 4 , Kehinde Ayooluwabomi Olokode 1 , Henry A Adeola 5
Affiliation  

Cadmium is a toxic metal and poses a high environmental risk to animals and humans, alike. It is thus pertinent to search for medicinal plants in protecting against cadmium toxicity. This study aims at investigating the ability of aqueous extract of Persea americana seeds (AEPA) in ameliorating the toxic effects of cadmium in the kidneys of cadmium-exposed Wistar rats. Male Wistar rats were grouped into five, of six animals each. Different groups of animals received normal saline (control group), 200 mg/kg body weight AEPA, 400 mg/kg AEPA, and standard drug, Livolin Forte, respectively. A last group of animals was left untreated. To induce toxicity, all animals, except the control group, were exposed to cadmium (200 mg/L, as CdCl2) in their main drinking water for 21 days. Biochemical analysis of serum kidney markers, oxidative stress and antioxidant status, as well as anti-inflammatory activities, was done using standard methods and kits. In silico analysis was performed on phytochemicals reported to be abundant in AEPA. Treatment with 400 mg/kg AEPA significantly reversed (P ≤ 0.05) the adverse effect of cadmium on serum creatinine, urea, uric acid and blood urea nitrogen, and restored (P ≤ 0.05) antioxidant status, evidenced by its significant effect on superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, reduced glutathione, and lipid peroxidation activities. AEPA, at 400 mg/kg also exhibited significant anti-inflammatory effects, which was shown by reduced interleukin-2 and tumour necrosis factor α activities. Molecular docking of phytochemicals with the selected protein target also confirmed the therapeutic potential of AEPA. The study concluded that aqueous extract of AEPA protects against cadmium-induced kidney toxicity and inflammation.



中文翻译:

Persea americana 种子水提取物对镉诱导的 Wistar 大鼠肾毒性的肾保护和抗炎作用

镉是一种有毒金属,对动物和人类都有很高的环境风险。因此,寻找药用植物以防止镉毒性是相关的。本研究旨在调查美洲波斯菊种子水提取物( AEPA) 改善镉暴露的 Wistar 大鼠肾脏中镉的毒性作用的能力。雄性 Wistar 大鼠被分成五组,每组六只。不同组的动物分别接受生理盐水(对照组)、200 mg/kg体重的AEPA、400 mg/kg AEPA和标准药物Livolin Forte。最后一组动物未经治疗。为了诱导毒性,除对照组外,所有动物均暴露于镉(200 mg/L,如 CdCl 2) 在他们的主要饮用水中 21 天。使用标准方法和试剂盒对血清肾脏标志物、氧化应激和抗氧化状态以及抗炎活性进行生化分析。对据报道在 AEPA 中含量丰富的植物化学物质进行了计算机分析。400 mg/kg AEPA处理显着逆转(P≤0.05  )镉对血清肌酐、尿素、尿酸和血尿素氮的不良影响,并恢复(P ≤ 0.05) 抗氧化状态,其对超氧化物歧化酶、过氧化氢酶、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶、还原型谷胱甘肽和脂质过氧化活性有显着影响。400 mg/kg 的 AEPA 也表现出显着的抗炎作用,这表现为白细胞介素 2 和肿瘤坏死因子 α 活性降低。植物化学物质与选定蛋白质靶标的分子对接也证实了 AEPA 的治疗潜力。该研究得出结论,AEPA 的水提取物可防止镉引起的肾脏毒性和炎症。

更新日期:2021-08-10
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