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Anthocyanins in Lycium ruthenicum Murray reduce nicotine withdrawal-induced anxiety and craving in mice
Neuroscience Letters ( IF 2.5 ) Pub Date : 2021-08-10 , DOI: 10.1016/j.neulet.2021.136152
Jing Luo 1 , Li-Hua Bian 1 , Zi-Wei Yao 1 , Xiao-Mei Wang 1 , Qiu-Yu Li 1 , Jian-You Guo 2 , Jin-Li Shi 1
Affiliation  

Lycium ruthenicum Murray is widely used in traditional Chinese medicine and is believed to have antimicrobial, antioxidant, and anti-fatigue effects. Anthocyanins are considered to be one of the main active components. The previous work by our research team found that the anthocyanins in Lycium ruthenicum extract (ALRM) produce a stable anti-anxiety effect. The mechanisms of action include reducing the level of corticotropin-releasing factor (CRF) as well as regulating extracellular signal-regulated kinase/mitogen activation, protein kinase (ERK/MAPK) pathways, and others, all of which are related to the mechanisms of nicotine addiction. To investigate the effects of ALRM on anxiety and craving behavior after nicotine withdrawal, the components of ALRM were analyzed using the UPLC-Orbitrap MS method. The effects of ALRM on anxiety behavior induced by nicotine withdrawal were investigated in mice using the elevated plus maze (EPM) and light–dark box (LDB) tests. The effects of ALRM on craving behavior after nicotine withdrawal were further investigated using the conditional place preference (CPP) test. The EPM and LDB tests demonstrated that ALRM could alleviate the anxiety behavior induced by nicotine withdrawal and reduce nicotine craving in mice. Based on the identified ALRM components, the network pharmacology method was used to predict the mechanism of ALRM alleviating anxiety after nicotine withdrawal in mice. It was speculated that ALRM was involved in the production and transmission of dopamine, choline, and other nervous system functions and exhibited a potential role in treating nicotine addiction.



中文翻译:

黑枸杞中的花青素可减少尼古丁戒断引起的小鼠焦虑和渴望

Lycium ruthenicum Murray 广泛用于中药,被认为具有抗菌、抗氧化和抗疲劳的作用。花青素被认为是主要的活性成分之一。我们课题组前期工作发现黑枸杞中的花青素提取物(ALRM)产生稳定的抗焦虑作用。作用机制包括降低促肾上腺皮质激素释放因子(CRF)水平以及调节细胞外信号调节激酶/丝裂原激活、蛋白激酶(ERK/MAPK)通路等,所有这些都与尼古丁成瘾。为了研究 ALRM 对尼古丁戒断后焦虑和渴望行为的影响,使用 UPLC-Orbitrap MS 方法分析了 ALRM 的成分。使用高架十字迷宫 (EPM) 和明暗盒 (LDB) 测试在小鼠中研究了 ALRM 对尼古丁戒断引起的焦虑行为的影响。使用条件位置偏好 (CPP) 测试进一步研究了 ALRM 对尼古丁戒断后渴望行为的影响。EPM 和 LDB 测试表明,ALRM 可以缓解尼古丁戒断引起的焦虑行为,减少小鼠对尼古丁的渴望。基于识别出的 ALRM 成分,采用网络药理学方法预测 ALRM 缓解小鼠尼古丁戒断后焦虑的机制。据推测,ALRM 参与了多巴胺、胆碱和其他神经系统功能的产生和传递,并在治疗尼古丁成瘾方面表现出潜在的作用。

更新日期:2021-08-25
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