Journal of Environmental Health Science and Engineering ( IF 3.4 ) Pub Date : 2021-08-09 , DOI: 10.1007/s40201-021-00716-8 Reza Ahmadkhaniha 1 , Faezeh Izadpanah 2 , Noushin Rastkari 2, 3
Background
1, 3-Butadiene is a famous industrial compound which occurs in gasoline and diesel exhaust and in cigarette smoke. Based on little evidence of carcinogenicity in laboratory animals and humans, it was classified as a probable carcinogen. The potential of exposure to gasoline and diesel exhaust containing these chemical compounds is very considerable in urban areas. According to studies, in estimation of workers’ chronic exposure with biological samples, analysis of the concentration of related hemoglobin adducts seems to give the most valid estimation of exposure.
Methods
This study designed to determine the level of chronic exposure in gas station workers and traffic policemen during routine work shift, by an appropriate biological marker. In this regards, 25 gas station workers, 25 policemen engaged in traffic control, and 25 occupationally non-exposed persons were studied. Blood samples were obtained after the work shift from each person. The level of selected hemoglobin adduct, 1- and 2-hydroxy-3-butenyl valine (MHBVal) was determined by using GC–MS after a modified Edman degradation and a further acetylation.
Results
There were significant differences among the mean concentrations of MHBVal in blood samples of gas station workers, policemen and occupationally non-exposed persons. The mean airborne levels of 1, 3-Butadiene over 60 days, differed significantly among different studied groups (ANOVA: p < 0.05 and Kruskal-Wallis test: p < 0.05).
Conclusion
There was a significant difference in MHBVal concentrations between job categories (p < 0.05 by ANOVA and Kruskal-Wallis test), and gas station workers and policemen were found to be probably the most exposed groups in this research.
中文翻译:
血红蛋白加合物作为长期暴露于低浓度 1, 3-丁二烯的重要标志物
背景
1, 3-丁二烯是一种著名的工业化合物,存在于汽油和柴油尾气以及香烟烟雾中。由于在实验动物和人类中几乎没有致癌性证据,它被归类为可能的致癌物。在城市地区,接触含有这些化合物的汽油和柴油废气的可能性非常大。根据研究,在估计工人长期接触生物样品时,分析相关血红蛋白加合物的浓度似乎可以提供最有效的接触估计。
方法
本研究旨在通过适当的生物标志物确定加油站工作人员和交警在常规轮班期间的慢性暴露水平。在这方面,对25名加油站工作人员、25名从事交通管制的警察和25名职业非接触者进行了研究。在每个人的工作轮班后获取血液样本。在改进的 Edman 降解和进一步乙酰化后,使用 GC-MS 测定选定的血红蛋白加合物、1-和 2-羟基-3-丁烯基缬氨酸 (MHBVal) 的水平。
结果
加油站工作人员、警察和职业非接触者血样中MHBVal的平均浓度存在显着差异。60 天内 1, 3-丁二烯的平均空气传播水平在不同研究组之间存在显着差异(ANOVA:p < 0.05 和 Kruskal-Wallis 检验:p < 0.05)。
结论
工作类别之间的 MHBVal 浓度存在显着差异(通过 ANOVA 和 Kruskal-Wallis 检验p < 0.05),并且发现加油站工人和警察可能是本研究中暴露最多的群体。