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Next-Generation Sequencing and Its Impacts on Entomological Research in Ecology and Evolution
Neotropical Entomology ( IF 1.8 ) Pub Date : 2021-08-10 , DOI: 10.1007/s13744-021-00895-x
Débora Pires Paula 1
Affiliation  

The advent of NGS-based methods has been profoundly transforming entomological research. Through continual development and improvement of different methods and sequencing platforms, NGS has promoted mass elucidation of partial or whole genetic materials associated with beneficial insects, pests (of agriculture, forestry and animal, and human health), and species of conservation concern, helping to unravel ecological and evolutionary mechanisms and characterizing survival, trophic interactions, and dispersal. It is shifting the scale of biodiversity and environmental analyses from individuals and biodiversity indicator species to the large-scale study of communities and ecosystems using bulk samples of species or a mixed “soup” of environmental DNA. As the NGS-based methods have become more affordable, complexity demystified, and specificity and sensitivity proven, their use in entomological research has spread widely. This article presents several examples on how NGS-based methods have been used in entomology to provide incentives to apply them when appropriate and to open our minds to the expected advances in entomology that are yet to come.



中文翻译:

下一代测序及其对生态学和进化昆虫学研究的影响

基于 NGS 的方法的出现已经深刻地改变了昆虫学研究。通过对不同方法和测序平台的不断开发和改进,NGS 促进了与有益昆虫、害虫(农业、林业和动物以及人类健康)和保护关注物种相关的部分或全部遗传材料的大规模阐明,帮助解开生态和进化机制,并表征生存、营养相互作用和扩散。它正在将生物多样性和环境分析的规模从个体和生物多样性指标物种转变为使用大量物种样本或环境 DNA 的混合“汤”对社区和生态系统进行大规模研究。随着基于 NGS 的方法变得更加实惠,复杂性变得神秘,并且特异性和敏感性得到证实,它们在昆虫学研究中的应用已经广泛传播。本文提供了几个示例,说明如何在昆虫学中使用基于 NGS 的方法,以提供激励措施,在适当的时候应用它们,并让我们对昆虫学尚未到来的预期进展敞开心扉。

更新日期:2021-08-10
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