As a by-product of pearl production, Pinctada martensii meat dregs have a high level of protein but cannot be fully utilized. In this study, P. martensii meat dregs were first hydrolyzed by three pepsin enzymes, resulting in neutral proteinase enzymatic hydrolysate that had a higher effect on stimulating the proliferation of MC3T3-E1 cells, and cell proliferation increases of 37.37 ± 0.03%. Subsequently, after purification of alcohol precipitation, ultrafiltration, and Superdex G-25 gel chromatography, five fractions were further separated and purified in which fraction ZP2 could effectively improve cell proliferation induced an increase of 43.95 ± 0.03% in MC3T3-E1 cells growth. Consequently, with the help of alkaline phosphatase and methyl thiazolyl tetrazolium assay, five novel peptides (FDNEGKGKLPEEY, FWDGRDGEVDGFK, VLQTDNDALGKAK, IVLDSGDGVTH, and MVAPEEHP) derived from fraction ZP2 with the strongest osteogenic activity were screened, and their sequences were identified using Orbitrap Fusion Lumos Tribrid Orbital liquid chromatography-mass spectrometry. Therefore, the research results demonstrated that P. martensii meat could be used as a promising material for producing food additives for improving osteoporosis.

中文翻译：

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马氏珠母贝肉渣促成骨细胞增殖肽的制备及鉴定

马氏珠母贝肉渣作为珍珠生产的副产品，蛋白质含量高，但不能充分利用。在这项研究中，P。马氏肉渣首先被三种胃蛋白酶水解，产生中性蛋白酶酶解产物，对刺激MC3T3-E1细胞增殖有较高的作用，细胞增殖增加37.37±0.03%。随后，经醇沉、超滤和Superdex G-25凝胶层析纯化后，进一步分离纯化5个组分，其中组分ZP2可有效提高细胞增殖，诱导MC3T3-E1细胞生长增加43.95±0.03%。因此，在碱性磷酸酶和甲基噻唑基四唑分析的帮助下，筛选了源自具有最强成骨活性的组分 ZP2 的五种新肽（FDNEGKGKLPEEY、FWDGRDGEVDGFK、VLQTDNDALGKAK、IVLDSGDGVTH 和 MVAPEEHP），并使用 Orbitrap Fusion Lumos Tribrid Orbital 液相色谱-质谱法鉴定它们的序列。因此，研究结果表明，P. martensii肉可用作生产改善骨质疏松症的食品添加剂的有前景的原料。