Abstract

Resveratrol is an important stilbene, initially identified from red wine possessing immense therapeutic, cosmeceutical and nutraceutical applications. In the present study, endophytic fungus Arcopilus aureus(#12VVLMP) which produces resveratrol extracellularly was selected as a candidate for epigenetic modulation using natural supplements, precursor feeding, chemical elicitors and co-culturing to enhance resveratrol production. The present study highlighted the role of natural supplements i.e. grape seed extract and grape skin extract which constitute grape pomace to enhance resveratrol production by 27.7 and 13.65% respectively. Co-culturing also impacted the resveratrol production by A. aureus, enhancing it by 9.4%. Chemical elicitors and precursor feeding did not induce significant enhancement in resveratrol production. Enhancement of anti-oxidant effect was also observed in the case of use of natural supplements assayed by DPPH and ABTS^• radical scavenging assays. Similarly anti-staphylococcal and anti-candida activities were potentially higher when natural supplements were used followed by co-culturing. These findings indicate that the use of natural supplement which is a by-product of wine industry may be used as a modulator of resveratrol production by A. aureus. This shall lead to a cost-effective fermentation process of resveratrol production, the global demand of which is continuously increasing.

摘要

白藜芦醇是一种重要的芪，最初是从红葡萄酒中发现的，具有巨大的治疗、药妆和保健品应用。在本研究中，选择在细胞外产生白藜芦醇的内生真菌Arcopilus aureus (#12VVLMP) 作为表观遗传调节的候选者，使用天然补充剂、前体喂养、化学诱导剂和共培养来增强白藜芦醇的产生。本研究强调了天然补充剂的作用，即构成葡萄渣的葡萄籽提取物和葡萄皮提取物，分别将白藜芦醇产量提高 27.7% 和 13.65%。共培养也影响了金黄色葡萄球菌的白藜芦醇生产，提高 9.4%。化学诱导剂和前体喂养不会显着提高白藜芦醇的产生。^{在使用天然补充剂的情况下，DPPH 和 ABTS •}自由基清除试验也观察到了抗氧化作用的增强。同样，当使用天然补充剂并随后进行共培养时，抗葡萄球菌和抗念珠菌的活性可能更高。这些发现表明，作为葡萄酒工业副产品的天然补充剂的使用可用作金黄色葡萄球菌生产白藜芦醇的调节剂。这将导致具有成本效益的白藜芦醇生产发酵工艺，全球对白藜芦醇的需求不断增加。