Cell destruction in Hashimoto's thyroiditis (HT) involves autoantibodies and cytotoxic T lymphocytes. Thyrocytes maintenance occurs by pro-apoptotic, anti-apoptotic and cell proliferation balance.

Objectives

To characterize factors related to the mechanisms of apoptosis and cell proliferation in thyroid cells and intrathyroid lymphocytic infiltrate in HT.

Methods

We assessed lymphocytic infiltrate and thyroid cells from HT and normal thyroid by immunohistochemical analysis of cell proliferation (Ki-67), antiproliferation (p27Kip1), pro-apoptosis (Fas, Fas-ligand, BID) and anti-apoptosis (MCL-1, BCL2) markers.

Results

Lymphocytic infiltrate presented BCL2 and MCL-1 higher expression, Ki-67 and p27kip1 balance. Thyrocytes exhibited Fas and FasL balance, higher BID expression; MCL-1, BCL-2, Ki-67 similar to the normal thyroid. T4 and higher lymphocytes BID expression were associated.

Conclusions

In lymphocytic infiltrate predominated anti-apoptosis in relation to pro-apoptosis except for BID. Thyrocytes presented pro-apoptosis and anti-apoptosis balance and cell proliferation similar to normal thyroid. T4-associated BID expression in HT lymphocytes suggests the influence of thyroid hormone as a signal to up-regulate the BID pro-apoptotic protein and thus increase lymphocytic apoptosis rates.

中文翻译：

---

甲状腺激素对促凋亡标志物 BID 表达的影响，尽管桥本氏甲状腺炎的甲状腺内淋巴细胞浸润中抗凋亡激活占主导地位

桥本甲状腺炎 (HT) 中的细胞破坏涉及自身抗体和细胞毒性 T 淋巴细胞。通过促凋亡、抗凋亡和细胞增殖平衡来维持甲状腺细胞。

目标

旨在表征与甲状腺细胞凋亡和细胞增殖以及HT中甲状腺内淋巴细胞浸润机制相关的因素。

方法

我们通过细胞增殖（Ki-67）、抗增殖（p27Kip1）、促凋亡（Fas、Fas-ligand、BID）和抗凋亡（MCL-1、 BCL2) 标记。

结果

淋巴细胞浸润表现为BCL2和MCL-1高表达，Ki-67和p27kip1平衡。甲状腺细胞呈现Fas和FasL平衡，BID表达较高；MCL-1、BCL-2、Ki-67与正常甲状腺相似。T4 和更高的淋巴细胞 BID 表达相关。

结论

除 BID 外，在与促细胞凋亡相关的淋巴细胞浸润中，抗细胞凋亡占主导地位。甲状腺细胞呈现出与正常甲状腺相似的促凋亡和抗凋亡平衡和细胞增殖。HT 淋巴细胞中与 T4 相关的 BID 表达表明甲状腺激素作为上调 BID 促凋亡蛋白的信号的影响，从而增加了淋巴细胞凋亡率。