Soybean, as a major oil crop, is one of the most widely planted crops in the world. Fusarium oxysporum causes soybean root rot, leading to great economic losses to soybean planting every year globally. Chemical fungicide for controlling soybean F. oxysporum diseases may cause environmental problems and has human health risks. Biological control methods avoid these shortcomings; however, few studies have focused on biocontrol of soybean diseases caused by F. oxysporum. Aiming at this problem, we obtained biocontrol bacteria against soybean F. oxysporum by plate confrontation method. The type of the strain with the highest biocontrol activity was identified by molecular biological methods, and then its biocontrol effects were verified through greenhouse experiments. One of our isolated strain named BS06 strain had the highest activity, which was identified as Bacillus subtilis. Our study showed that BS06 strain could effectively control soybean F. oxysporum disease and significantly reduce F. oxysporum to infect soybean roots. Compared with control and carbendazim treatments, BS06 treatment had higher root biomass, plant height, leaf chlorophyll content, stem base diameter and control efficiency. Our results indicated that BS06 could effectively protect soybean root (BS06 strain might produce substances to inhibit F. oxysporum), which was potentially useful for soybean planting.

中文翻译：

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枯草芽孢杆菌 BS06 菌株保护大豆根部免受尖孢镰刀菌感染

大豆作为主要的油料作物，是世界上种植最广泛的作物之一。尖孢镰刀菌引起大豆根腐病，每年给全球大豆种植造成巨大经济损失。用于控制大豆尖孢镰刀菌病害的化学杀菌剂可能会引起环境问题并危害人类健康。生物防治方法避免了这些缺点；然而，很少有研究关注由尖孢镰刀菌引起的大豆病害的生物防治。针对这一问题，我们获得了针对大豆尖孢镰刀菌的生防菌以板块对抗法。通过分子生物学方法确定生防活性最高的菌株类型，然后通过温室试验验证其生防效果。我们分离的菌株之一名为 BS06 菌株的活性最高，经鉴定为枯草芽孢杆菌。我们的研究表明，BS06 菌株可有效防治大豆尖孢镰刀菌病，并显着降低尖孢镰刀菌侵染大豆根部。与对照和多菌灵处理相比，BS06处理具有更高的根生物量、株高、叶片叶绿素含量、茎基直径和防治效率。我们的研究结果表明，BS06 可以有效地保护大豆根部（BS06 菌株可能会产生抑制尖孢镰刀菌的物质 ），这对大豆种植具有潜在的价值。