Circular RNAs (circRNAs) are a class of novel RNAs, and aberrant expression of circRNAs has been implicated in human diseases, including gastric cancer (GC). This study aimed to identify the mechanism of circRNA_0043691 in regulating the progression of GC. GSE141977 was downloaded from Gene Expression Omibus (http://www.ncbi.nlm.nih.gov/geo/). Differentially expressed circRNAs were obtained by R software. The expression levels of circRNA_0043691 in GC tissue and normal tissue were identified by quantitative real-time polymerase chain reaction (qRT-PCR). Knockdown of circRNA_0043691 was then constructed and verified by qRT-PCR. Cell viability, migration, and invasion capacity were determined by Cell Counting Kit-8 assay, Transwell migration, and invasion, respectively. Next, knockdown of miR-873-3p was constructed and co-cultured with circRNA_0043691 knockdown to identify whether knockdown of miR-873-3p could attenuate the circRNA_0043691 knockdown on GC cells proliferation, migration, and invasion. The relationship between miR-873-3p and circRNA_0043691 or GART was predicted by bioinformatics tools and verified by dual-luciferase reporter. A total of 211 circRNAs were significantly differentially expressed, including 143 remarkably downregulated circRNAs and 68 significantly upregulated circRNAs. CircRNA_0043691 was upregulated in GC tissue. Knockdown of circRNA_0043691 decreased cell viability, migration, and invasion in GC cells. CircRNA_0043691 has potential putative binding sites with miR-873-3p. Moreover, CircRNA_0043691 positively regulated GART expression by sponging miR-873-3p. Furthermore, knockdown of miR-591 could partially attenuate the si-circRNA_0043691 on the GART expression. GART was upregulated in GC tissue and knockdown of GART could inhibit GC cells proliferation and invasion. Knockdown of circRNA_0043691 delayed the progression of GC via modulating the miR-873-3p-GART axis.

环状 RNA (circRNA) 是一类新型 RNA，circRNA 的异常表达与人类疾病有关，包括胃癌 (GC)。本研究旨在确定circRNA_0043691调控GC进展的机制。GSE141977 是从 Gene Expression Omibus (http://www.ncbi.nlm.nih.gov/geo/) 下载的。通过R软件获得差异表达的circRNA。通过定量实时聚合酶链反应（qRT-PCR）鉴定 circRNA_0043691 在 GC 组织和正常组织中的表达水平。然后构建了 circRNA_0043691 的敲低并通过 qRT-PCR 验证。细胞活力、迁移和侵袭能力分别通过 Cell Counting Kit-8 测定、Transwell 迁移和侵袭测定。下一个，构建了敲低 miR-873-3p 并与敲低 circRNA_0043691 共培养，以确定敲低 miR-873-3p 是否可以减弱 circRNA_0043691 敲低对 GC 细胞增殖、迁移和侵袭的影响。通过生物信息学工具预测miR-873-3p与circRNA_0043691或GART之间的关系，并通过双荧光素酶报告基因进行验证。共有 211 个 circRNA 显着差异表达，其中 143 个显着下调的 circRNA 和 68 个显着上调的 circRNA。CircRNA_0043691 在 GC 组织中上调。circRNA_0043691 的敲低降低了 GC 细胞中的细胞活力、迁移和侵袭。CircRNA_0043691 与 miR-873-3p 具有潜在的推定结合位点。此外，CircRNA_0043691 通过海绵化 miR-873-3p 正向调节 GART 表达。此外，敲低 miR-591 可以部分减弱 GART 表达上的 si-circRNA_0043691。GART在GC组织中上调，敲低GART可抑制GC细胞增殖和侵袭。circRNA_0043691 的敲除通过调节 miR-873-3p-GART 轴延缓了 GC 的进展。