In this study, three active ingredients, namely, gallic acid, orientin and orientin-2″-O-β-l-galactoside, in Chinese medicine compound antibacterial gels (CMCAGs) were identified and quantified by high-performance thin-layer chromatography (HPTLC). The thin-layer plate was a polyamide film plate, and the deploying agent was dichloromethane‒butanone‒methanol‒formic acid (6:0.5:2:0.6, V/V). The scanning wavelength was 280 nm for the determination of gallic acid and 360 nm for the determination of orientin and orientin-2″-O-β-l-galactoside. Under the deploying conditions, the R_F values of gallic acid, orientin and orientin-2″-O-β-l-galactoside were 0.37 ± 0.02, 0.51 ± 0.03 and 0.65 ± 0.04, respectively. The linearity ranges were 0.089‒0.785 μg, 0.026‒0.209 μg and 0.082‒0.575 μg with linear correlation coefficient (r) of 0.9957, 0.9950 and 0.9953, which indicated a good linear relationship. The average recovery rates were 99.94%, 100.92% and 98.82% with percent relative standard deviations of 2.67%, 3.70% and 3.69%, respectively. The developed method was simple and accurate for the simultaneous qualitative and quantitative analyses of the three components in the CMCAG.

本研究通过高效薄层色谱法对中药复方抗菌凝胶（CMCAGs）中的三种活性成分，即没食子酸、东方素和东方素-2″ -O - β- l-半乳糖苷进行了鉴定和定量。 HPTLC）。薄层板为聚酰胺薄膜板，展开剂为二氯甲烷-丁酮-甲醇-甲酸（6：0.5：2：0.6，V/V）。测定没食子酸的扫描波长为 280 nm，测定orientin 和 orientin -2" -O - β- l-半乳糖苷的扫描波长为 360 nm 。下的部署条件下，- [R _˚F没食子酸的值，荭和荭-2“ - ö -β-升-半乳糖苷分别为 0.37 ± 0.02、0.51 ± 0.03 和 0.65 ± 0.04。线性范围为0.089-0.785 μg、0.026-0.209 μg和0.082-0.575 μg，线性相关系数( r )分别为0.9957、0.9950和0.9953，表明线性关系良好。平均回收率为99.94%、100.92%和98.82%，相对标准偏差百分比分别为2.67%、3.70%和3.69%。开发的方法简单而准确，可同时对 CMCAG 中的三个组分进行定性和定量分析。