Introduction of biofuels to the fuel matrix poses new questions and challenges. This study presents the behavior of microbial growth on physicochemical properties in different kinds of biodiesel/diesel mixtures that are produced from raw materials. A variety of fatty acid methyl ester samples were produced from raw materials such as sunflower oil, soybean oil, pomace oil, castor oil, palm oil and used frying oil. After these samples were produced they were mixed with conventional automotive diesel at concentrations of 10 and 20% v/v in respect with biodiesel. Also similar blends were prepared with the above produced methyl esters differentiating in which the antioxidant TBHQ had been added previously in concentration 0.1%. The outcomes indicated that in mixtures of methyl esters with an augmented content of saturated fatty acids such as palm methyl esters and monounsaturated fatty acids (i.e. pomace methyl esters) there was appearance of microbial growth in the fuel phase shown more quickly while the mixtures with TBHQ showed reduction in microbial load. Furthermore, in samples with a large augmentation in the number of acidity, the microbial load detected was negligible or zero. Samples with a zero or decreased microbial activity indicated lower pH values in the water phase.

将生物燃料引入燃料基质提出了新的问题和挑战。本研究展示了微生物生长对由原材料生产的不同种类的生物柴油/柴油混合物的理化特性的行为。以葵花油、大豆油、果渣油、蓖麻油、棕榈油和用过的煎炸油为原料，生产了多种脂肪酸甲酯样品。生产这些样品后，将它们与传统汽车柴油以相对于生物柴油的 10% 和 20% v/v 的浓度混合。还用上述生产的甲酯制备了类似的共混物，不同之处在于其中预先以0.1%的浓度添加了抗氧化剂TBHQ。结果表明，在饱和脂肪酸含量增加的甲酯混合物中，如棕榈甲酯和单不饱和脂肪酸（即果渣甲酯），燃料阶段出现微生物生长的迹象更快，而与 TBHQ 的混合物显示微生物负荷减少。此外，在酸度数量大幅增加的样品中，检测到的微生物负荷可以忽略不计或为零。微生物活性为零或降低的样品表明水相中的 pH 值较低。检测到的微生物负荷可以忽略不计或为零。微生物活性为零或降低的样品表明水相中的 pH 值较低。检测到的微生物负荷可以忽略不计或为零。微生物活性为零或降低的样品表明水相中的 pH 值较低。