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Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
Journal of Analytical Methods in Chemistry ( IF 2.6 ) Pub Date : 2021-08-09 , DOI: 10.1155/2021/1857464
Vu Thi Thu 1, 2 , Ngo Thi Hai Yen 1 , Nguyen Thi Ha Ly 3
Affiliation  

Aims. The purpose of this study was to evaluate the protective effect of liquiritin (LIQ) from Radix Glycyrrhizae on cardiac mitochondria against hypoxia/reoxygenation (HR) injury. Methods. H9C2 cells were subject to the HR model. LIQ purified from Radix Glycyrrhizae (purity > 95%) was administrated to reoxygenation period. Cell viability, mitochondrial mass, mitochondrial membrane potential, reactive oxygen species, and mitochondrial Ca2⁺ level were then assessed by using Cell Counting kit-8 and suitable fluorescence probe kits. Results. LIQ administration remarkably reduced the rate of HR damage via increasing H9C2 cell viability level and preserving mitochondria after HR. Particularly, 60 μM of LIQ posthypoxic treatment markedly reduced cell death in HR-subjected H9C2 cell groups (). Interestingly, posthypoxic treatment of LIQ significantly prevented the loss of mitochondrial membrane potential, the decrease in mitochondrial mass, the increase in reactive oxygen species production, and the elevation of mitochondrial Ca2⁺ level in HR-treated H9C2 cells. Conclusion. The present study provides for the first time the cardioprotective of LIQ posthypoxic treatment via reducing H9C2 cell death and protecting cardiac mitochondria against HR damage.

中文翻译:

甘草中的甘草苷保护心脏线粒体免受缺氧/复氧损伤

目标。本研究的目的是评估甘草中的甘草苷(LIQ)对心脏线粒体对缺氧/复氧(HR)损伤的保护作用方法。H9C2细胞受HR模型的影响。从甘草中纯化的LIQ (纯度> 95%)被施用至复氧期。然后使用 Cell Counting kit-8 和合适的荧光探针试剂盒评估细胞活力、线粒体质量、线粒体膜电位、活性氧和线粒体 Ca 2 + 水平。结果。LIQ 给药通过提高 H9C2 细胞活力水平和在 HR 后保留线粒体,显着降低了 HR 损伤率。特别是,60 μM LIQ 缺氧后处理显着减少 HR 处理的 H9C2 细胞组的细胞死亡()。有趣的是,LIQ 的缺氧处理显着防止了 HR 处理的 H9C2 细胞中线粒体膜电位的丧失、线粒体质量的减少、活性氧物质的产生增加以及线粒体 Ca 2 + 水平的升高。结论。本研究首次通过减少 H9C2 细胞死亡和保护心脏线粒体免受 HR 损伤提供了 LIQ 缺氧后治疗的心脏保护作用。
更新日期:2021-08-09
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