Conjugation of various reagents to antibodies has long been an elegant way to combine the superior binding features of the antibody with other desired but non-natural functions. Applications range from labels for detection in different analytical assays to the creation of new drugs by conjugation to molecules which improves the pharmaceutical effect. In many of these applications, it has been proven advantageous to control both the site and the stoichiometry of the conjugation to achieve a homogeneous product with predictable, and often also improved, characteristics. For this purpose, many research groups have, during the latest decade, reported novel methods and techniques, based on small molecules, peptides, and proteins with inherent affinity for the antibody, for site-specific conjugation of antibodies. This review provides a comprehensive overview of these methods and their applications and also describes a historical perspective of the field.

中文翻译：

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基于亲和力的抗体位点特异性偶联方法

长期以来，将各种试剂与抗体结合一直是将抗体的卓越结合特性与其他所需但非天然功能相结合的优雅方式。应用范围从用于在不同分析测定中进行检测的标记到通过与分子缀合以提高药物效果来创造新药物。在许多这些应用中，已证明控制缀合的位点和化学计量是有利的，以获得具有可预测且通常还改进的特性的均质产品。为此，在最近十年中，许多研究小组报告了基于小分子、肽和对抗体具有内在亲和力的蛋白质的新方法和技术，用于位点特异性抗体偶联。