Tissue-engineered skin, as a promising skin substitute, can be used for in vitro skin research and skin repair. However, most of research on tissue-engineered skin tend to ignore the rete ridges (RRs) microstructure, which enhances the adhesion between dermis and epidermis and provides a growth environment for epidermal stem cells. Here, we prepared and characterized photocurable gelatin methacrylated (GelMA) and poly(ethylene glycol) diacrylate (PEGDA) co-network hydrogels with different concentrations. Using a UV curing 3D printer, resin molds were designed and fabricated to create three-dimensional micropatterns and replicated onto GelMA-PEGDA scaffolds. Human keratinocytes (HaCaTs) and human skin fibroblasts (HSFs) were co-cultured on the hydrogel scaffold to prepare tissue-engineered skin. The results showed that 10%GelMA-2%PEGDA hydrogel provides the sufficient mechanical properties and biocompatibility to prepare a human skin model with RRs microstructure, that is, it presents excellent structural support, suitable degradation rate, good bioactivity and is suitable for long-term culturing. Digital microscope image analyses showed the micropattern was well-transferred onto the scaffold surface. Both in vitro and in vivo experiments confirmed the formation of the epidermal layer with undulating microstructure. In wound healing experiments, hydrogel can significantly accelerate wound healing. This study provides a simple and powerful way to mimic the structures of human skin and can make a contribution to skin tissue engineering and wound healing.

组织工程皮肤作为一种很有前途的皮肤替代品，可用于体外皮肤研究和皮肤修复。然而，大多数关于组织工程皮肤的研究往往忽略了网脊（RRs）微观结构，它增强了真皮和表皮之间的粘附力，为表皮干细胞提供了生长环境。在这里，我们制备并表征了不同浓度的光固化明胶甲基丙烯酸酯（GelMA）和聚（乙二醇）二丙烯酸酯（PEGDA）共网络水凝胶。使用 UV 固化 3D 打印机，设计和制造树脂模具以创建 3D 微图案并复制到 GelMA-PEGDA 支架上。人类角质形成细胞 (HaCaTs) 和人类皮肤成纤维细胞 (HSFs) 在水凝胶支架上共培养以制备组织工程皮肤。结果表明，10%GelMA-2%PEGDA水凝胶为制备具有RRs微结构的人体皮肤模型提供了足够的力学性能和生物相容性，即具有优良的结构支撑、合适的降解速率、良好的生物活性，适合长期使用。术语培养。数字显微镜图像分析显示微图案很好地转移到支架表面。体外和体内实验均证实了具有起伏微结构的表皮层的形成。在伤口愈合实验中，水凝胶可以显着加速伤口愈合。这项研究提供了一种简单而强大的方法来模拟人体皮肤的结构，并且可以为皮肤组织工程和伤口愈合做出贡献。