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Mesenchymal Stem Cells Resist Mechanical Confinement through the Activation of the Cortex during Cell Division
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2021-08-08 , DOI: 10.1021/acsbiomaterials.1c00862
Chuanchuan Lin 1 , Ye He 1, 2 , Kun Xu 1 , Qian Feng 1 , Xuan Li 1 , Songyue Zhang 1 , Ke Li 1 , Ruqing Bai 3 , Hong Jiang 4 , Kaiyong Cai 1, 5
Affiliation  

The mechanical properties of the natural extracellular matrix (ECM) change extensively, but these specific properties provide a relatively stable environment for resident cells. Although the effect of matrix stiffness on cell functions has been widely studied, the molecular mechanism was still not fully understood. Matrix stiffening is a common phenomenon in tissue damaging processes. To explore the effect of the increase in local matrix stiffness on cell behaviors, a three-dimensional (3D) cell culture system with a tunable modulus but constant other physical parameters was constructed by the alginate hydrogel with different molecular weights and cross-linking degrees. By using this culture system, the transcriptome response of mesenchymal stem cells (MSCs) to matrix stiffness was explored. Furthermore, a finite element model was developed to simulate the interaction between cells and the matrix. Results revealed that the increased matrix stiffness promoted the proliferation-related signaling of MSCs, and this process depended on the increased cortex tension caused by the activation of RAS and myosin II.

中文翻译:

间充质干细胞通过在细胞分裂过程中激活皮层来抵抗机械限制

天然细胞外基质 (ECM) 的机械特性发生广泛变化,但这些特定特性为常驻细胞提供了相对稳定的环境。尽管基质刚度对细胞功能的影响已被广泛研究,但其分子机制仍未完全了解。基质硬化是组织损伤过程中的常见现象。为了探索局部基质刚度的增加对细胞行为的影响,通过具有不同分子量和交联度的海藻酸盐水凝胶构建了具有可调模量但其他物理参数恒定的三维 (3D) 细胞培养系统。通过使用该培养系统,研究了间充质干细胞 (MSC) 对基质刚度的转录组反应。此外,开发了一个有限元模型来模拟细胞和基质之间的相互作用。结果表明,基质硬度的增加促进了 MSCs 的增殖相关信号传导,这一过程依赖于由 RAS 和肌球蛋白 II 的激活引起的皮层张力增加。
更新日期:2021-09-13
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