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FISH analysis of brain smears obtained at intraoperative diagnosis – An accurate and fast method to detect 1p/19q-codeletion in gliomas
Journal of Clinical Neuroscience ( IF 2 ) Pub Date : 2021-08-07 , DOI: 10.1016/j.jocn.2021.07.043
Joanne Brown 1 , Sally Byatt 1 , Tayyaba Khan 1 , Luke St Heaps 2 , Mark Dexter 3 , Najmun Nahar 4 , Mark Wong 5 , King Tan 6 , Winny Varikatt 7
Affiliation  

The importance of molecular testing of gliomas is highlighted in the 2016 revised 4th edition of the WHO Classification of Tumours of the Central Nervous System, which applies an integrated diagnosis of histological and molecular features. In this classification system, oligodendrogliomas (ODG) are defined as IDH-mutant and 1p/19q-codeleted. Fluorescence in situ hybridization (FISH) analysis of formalin-fixed paraffin-embedded (FFPE) tissue is a standard method of determining 1p/19q-codeletion. However, it has several disadvantages, including requiring lengthy pretreatment, truncation artefact and lack of on-site access in many centers. In an effort to address these issues, we analysed FISH performed on smears obtained at intraoperative frozen section on 51 gliomas and compared this to FISH performed on subsequent FFPE sections. Four cases were excluded due to uninterpretable FISH results. Of the remaining 47 cases, 17 were concordant for 1p/19q-codeletion, 29 were concordant for lack of 1p/19q-codeletion, and 1 was discordant with 1p/19q-codeletion found on FFPE tissue but not on intraoperative smears. The discordant case was most likely due to sampling error, as the frozen section had not shown definite tumor. The FISH results on intraoperative smears were received within 24–48 h after the sample was collected, compared with 3–4 days for FFPE tissue. FISH on smears obtained at intraoperative frozen section is an accurate and fast method for determining 1p/19q-codeletion.



中文翻译:

术中诊断获得的脑涂片的 FISH 分析——一种准确快速检测胶质瘤 1p/19q 编码缺失的方法

2016 年修订的第 4 版世界卫生组织中枢神经系统肿瘤分类强调了胶质瘤分子检测的重要性,该分类应用了组织学和分子特征的综合诊断。在这个分类系统中,少突胶质细胞瘤 (ODG) 被定义为 IDH 突变和 1p/19q 编码。福尔马林固定石蜡包埋 (FFPE) 组织的荧光原位杂交 (FISH) 分析是确定 1p/19q 编码缺失的标准方法。然而,它有几个缺点,包括需要冗长的预处理、截断伪影和许多中心缺乏现场访问。为了解决这些问题,我们分析了对 51 个神经胶质瘤术中冰冻切片获得的涂片进行的 FISH,并将其与在随后的 FFPE 切片上进行的 FISH 进行了比较。由于无法解释的 FISH 结果,四例被排除在外。在其余 47 例中,17 例与 1p/19q 编码缺失一致,29 例与 1p/19q 编码缺失一致,1 例与 FFPE 组织上发现的 1p/19q 编码缺失不一致,但在术中涂片上没有。不一致的情况很可能是由于取样错误,因为冷冻切片没有显示出明确的肿瘤。术中涂片的 FISH 结果在收集样本后 24-48 小时内收到,而 FFPE 组织则需要 3-4 天。术中冰冻切片涂片 FISH 是确定 1p/19q 编码缺失的一种准确、快速的方法。和 1 与 FFPE 组织上发现的 1p/19q 编码缺失不一致,但在术中涂片上没有。不一致的情况很可能是由于取样错误,因为冷冻切片没有显示出明确的肿瘤。术中涂片的 FISH 结果在收集样本后 24-48 小时内收到,而 FFPE 组织则需要 3-4 天。术中冰冻切片涂片 FISH 是确定 1p/19q 编码缺失的一种准确、快速的方法。和 1 与 FFPE 组织上发现的 1p/19q 编码缺失不一致,但在术中涂片上没有。不一致的情况很可能是由于取样错误,因为冷冻切片没有显示出明确的肿瘤。术中涂片的 FISH 结果在收集样本后 24-48 小时内收到,而 FFPE 组织则需要 3-4 天。术中冰冻切片涂片 FISH 是确定 1p/19q 编码缺失的一种准确、快速的方法。

更新日期:2021-08-09
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