Statement of problem

The effect of aging and the surface treatment of implants on osseointegration needs to be evaluated.

Purpose

The purpose of this in vitro study was to evaluate the effects of aging and the surface treatment of titanium with ultraviolet (UV) radiation and fibroblast growth factor (FGF) on hydrophilicity and cell growth and thus on osseointegration.

Material and methods

A total of 28 specimens were divided into 2 groups to measure hydrophilicity (n=14) and cell growth (n=14). Each group was further divided into 4 groups according to surface modification. These include the control group (CG) (nascent specimens), aged group (AG) (nascent specimens aged for 4 weeks), photofunctionalized group (PG) (aged specimens UV-A treated), and mimed group (MG) (aged specimens UV-A and FGF2 treated). The PG and MG specimens were treated with UV-A light for 40 minutes. The biomimetic surface modification was performed for MG. Hydrophilicity was measured by using the contact angle in relation to the surface of titanium disks with the help of a drop shape analyzing device (KRUSS), and cell growth was measured by calculating the number of stem cells per cm² with the help of a scanning electron microscope (SEM). The data obtained were subjected to statistical analysis with a statistical software program (α=.05).

Results

The lowest contact angle values were found in PG (13.52 ±0.90 degrees) and the highest in AG (70.54 ±1.72 degrees). The highest number of cells per cm² (2880 ±99.33) were found for MG, and the lowest number of cells per cm² (760 ±9.17) for AG.

Conclusions

Aging decreased the hydrophilicity and cell adhesion, migration, and growth on the titanium surface. UV treatment improved the hydrophilicity, cell adhesion, migration, and growth for both CG and AG. FGF2 treatment increased the cell adhesion, migration, and growth for CG, AG, and PG.

中文翻译：

---

钛种植体表面改性对改善骨整合的影响：一项体外研究

问题陈述

需要评估老化和植入物表面处理对骨整合的影响。

目的

这项体外研究的目的是评估老化和钛表面处理的紫外线 (UV) 辐射和成纤维细胞生长因子 (FGF) 对亲水性和细胞生长以及骨整合的影响。

材料与方法

将总共​​ 28 个样本分为 2 组以测量亲水性 (n=14) 和细胞生长 (n=14)。每组根据表面改性进一步分为4组。这些包括对照组（CG）（新生标本）、老化组（AG）（老化4周的新生标本）、光功能化组（PG）（UV-A处理的老化标本）和模拟组（MG）（老化标本UV-A 和 FGF2 处理）。PG 和 MG 样品用 UV-A 光处理 40 分钟。对MG进行了仿生表面改性。亲水性是通过使用接触角相对于钛的磁盘的表面与一个液滴形状分析装置（KRUSS）的帮助下测定，细胞生长通过计算干细胞的每平方厘米数来测量²在扫描电子显微镜 (SEM) 的帮助下。使用统计软件程序对获得的数据进行统计分析（α=.05）。

结果

PG 中的接触角值最低（13.52 ±0.90 度），AG 中的接触角最高（70.54 ±1.72 度）。每平方厘米的细胞数量最多²（2880±99.33）被发现为MG，和最低数每厘米细胞²（760±9.17）为AG。

结论

老化降低了钛表面的亲水性和细胞粘附、迁移和生长。紫外线处理改善了 CG 和 AG 的亲水性、细胞粘附、迁移和生长。FGF2 处理增加了 CG、AG 和 PG 的细胞粘附、迁移和生长。