Abstract

In this investigation, arsenic (As) and nickel (Ni) removal ability of fungus Trichoderma lixii CR700 have been explored. During the batch study, it showed efficient removal ability for As and Ni which was 94.7 and 68% respectively at 10 mgL⁻¹ and 144 h after incubation, however, increasing concentration from 10 to 500 mgL⁻¹ of As/Ni affected the removal ability of the fungus. The optimum pH, temperature and time for removal of As and Ni were 8.0, 28 °C and 240 h after inoculation. T. lixii CR700 also exhibited significant removal ability under the presence of salts, anions, EDTA and heavy metals. As and Ni removal was mainly happened by accumulation, where glutathione and thiol content might be played an important role in addition to surface sorption mechanism as confirmed from FTIR. T. lixii CR700 showed multifarious morphological and biochemical responses to deal As and Ni toxicity. Toxicity potential of fungal treated solution of As/Ni was diminished as confirmed from superior growth and high germination rate of Vigna radiata seeds in fungus treated As/Ni solution. Therefore, T. lixii CR700 can be potentially exploited for the management of As/Ni polluted aqueous system in safe and sustainable way.

摘要

在本次调查中，探索了真菌Trichoderma lixii CR700 对砷 (As) 和镍 (Ni) 的去除能力。^{在批量研究期间，它显示出对 As 和 Ni 的有效去除能力，在孵育后 10 mgL -1}和 144 h分别为 94.7% 和 68% ，然而，将 As/Ni 浓度从 10 增加到 500 mgL ^-1会影响去除真菌的能力。去除As和Ni的最佳pH、温度和时间分别为接种后8.0、28 ℃和240 h。T. lixiiCR700 在盐、阴离子、EDTA 和重金属的存在下也表现出显着的去除能力。As和Ni的去除主要通过积累发生，除了FTIR证实的表面吸附机制外，谷胱甘肽和硫醇含量可能发挥重要作用。T. lixii CR700 对处理 As 和 Ni 毒性表现出多种形态和生化反应。真菌处理的 As/Ni 溶液的毒性潜力降低，这可以从经过真菌处理的 As/Ni 溶液中的Vigna radiata种子的优异生长和高发芽率证实。因此，T. lixii CR700 可潜在地用于以安全和可持续的方式管理 As/Ni 污染的水系统。