The neuroprotective activity of 2-heptyl-3-hydroxy-4(1H)-quinolone (compound 1) was evaluated using the neurotoxicity of glutamate in the HT22 cell line. Compound 1, known as a signal molecule of the bacterial quorum-sensing system, protects neuronal cells from glutamate-induced neurotoxicity by inhibiting cellular Ca²⁺ uptake and glutamate-triggered ROS accumulation. MAPK signaling pathway inhibition by compound 1 was evaluated by immunoblotting the phosphorylation status of the proteins. Furthermore, pro-apoptotic protein levels and AIF translocation to the nucleus were found to be reduced by compound 1. In conclusion, compound 1 showed neuroprotective effects by inhibiting apoptotic neuronal cell death.

2-heptyl-3-hydroxy-4(1H)-quinolone（化合物 1）的神经保护活性使用谷氨酸在 HT22 细胞系中的神经毒性进行评估。化合物 1，被称为细菌群体感应系统的信号分子，通过抑制细胞 Ca ²⁺摄取和谷氨酸引发的 ROS 积累，保护神经元细胞免受谷氨酸诱导的神经毒性。通过对蛋白质的磷酸化状态进行免疫印迹评估化合物 1 对 MAPK 信号通路的抑制。此外，发现化合物 1 降低了促凋亡蛋白水平和 AIF 向细胞核的易位。总之，化合物 1 通过抑制凋亡神经元细胞死亡显示出神经保护作用。