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Determination of the Chemical Composition, Antioxidant, and Enzyme Inhibitory Activity of Onosma mollis DC
Journal of Chemistry ( IF 3 ) Pub Date : 2021-08-07 , DOI: 10.1155/2021/5405365 Arzuhan Sihoglu Tepe 1
Journal of Chemistry ( IF 3 ) Pub Date : 2021-08-07 , DOI: 10.1155/2021/5405365 Arzuhan Sihoglu Tepe 1
Affiliation
Onosma species have long been used traditionally for respiratory tract infections, abdominal pain, wound treatment, burns, and constipation. This study aims to investigate the chemical composition and in vitro antioxidant and enzyme inhibitory activities of ethyl acetate (EtOAc), methanol (MeOH), and water extracts of Onosma mollis DC. MeOH extract was richer in both phenolics and flavonoids than other extracts (44.06 mg GAEs/g and 41.57 mg QEs/g, respectively). The findings obtained from the results of the chromatographic analysis also supported the results of the spectrophotometric analysis. The MeOH extract was the richest in terms of most of the phytochemicals screened. Apigenin 7-glucoside, luteolin 7-glucoside, rosmarinic acid, vanillic acid, and pinoresinol were over 1000.0 μg/g in MeOH extract. The extract in question showed the highest activity in phosphomolybdenum, DPPH, and ABTS radical scavenging and CUPRAC and FRAP reducing power activity assays (2.01, 3.33, 2.30, 1.48, and 0.79 mg/ml, respectively). The water extract presented the highest activity in the ferrous ion chelating assay (1.01 mg/ml). While EtOAc extract showed high activity in acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase inhibitory activity tests (1.11, 1.49, and 1.07 mg/ml, respectively), MeOH extract showed significant efficacy in tyrosinase and α-amylase inhibitory activity assays (2.94 and 2.08 mg/ml, respectively). There was a high correlation between the total phenolics/flavonoids of the extracts and their antioxidant activities (correlation coefficients were over 0.9). In addition, the phytochemicals mentioned above were found to contribute significantly to the antioxidant activity. It was concluded that a more detailed analysis should be done to determine the compounds responsible for the enzyme inhibitory activities of the extracts.
中文翻译:
确定 Onosma mollis DC 的化学成分、抗氧化和酶抑制活性
Onosma物种长期以来一直用于呼吸道感染、腹痛、伤口治疗、烧伤和便秘。本研究旨在研究乙酸乙酯 (EtOAc)、甲醇 (MeOH) 和Onosma mollis水提取物的化学成分和体外抗氧化和酶抑制活性。直流。MeOH 提取物比其他提取物富含酚类和黄酮类化合物(分别为 44.06 mg GAEs/g 和 41.57 mg QEs/g)。从色谱分析的结果中获得的发现也支持了分光光度分析的结果。就大多数筛选的植物化学物质而言,MeOH 提取物是最丰富的。芹菜素 7-葡萄糖苷、木犀草素 7-葡萄糖苷、迷迭香酸、香草酸和松脂醇超过 1000.0 μ克/克甲醇提取物中。所讨论的提取物在磷钼、DPPH 和 ABTS 自由基清除以及 CUPRAC 和 FRAP 还原能力活性测定中显示出最高的活性(分别为 2.01、3.33、2.30、1.48 和 0.79 mg/ml)。水提取物在亚铁离子螯合试验中表现出最高的活性 (1.01 mg/ml)。虽然 EtOAc 提取物在乙酰胆碱酯酶 (AChE)、丁酰胆碱酯酶 (BChE) 和α-葡萄糖苷酶抑制活性测试中显示出高活性(分别为 1.11、1.49和 1.07 mg/ml),但 MeOH 提取物在酪氨酸酶和α-淀粉酶抑制活性测定(分别为 2.94 和 2.08 毫克/毫升)。提取物的总酚类/黄酮类化合物与其抗氧化活性之间存在高度相关性(相关系数超过 0.9)。此外,发现上述植物化学物质对抗氧化活性有显着贡献。结论是应该进行更详细的分析以确定负责提取物酶抑制活性的化合物。
更新日期:2021-08-09
中文翻译:
确定 Onosma mollis DC 的化学成分、抗氧化和酶抑制活性
Onosma物种长期以来一直用于呼吸道感染、腹痛、伤口治疗、烧伤和便秘。本研究旨在研究乙酸乙酯 (EtOAc)、甲醇 (MeOH) 和Onosma mollis水提取物的化学成分和体外抗氧化和酶抑制活性。直流。MeOH 提取物比其他提取物富含酚类和黄酮类化合物(分别为 44.06 mg GAEs/g 和 41.57 mg QEs/g)。从色谱分析的结果中获得的发现也支持了分光光度分析的结果。就大多数筛选的植物化学物质而言,MeOH 提取物是最丰富的。芹菜素 7-葡萄糖苷、木犀草素 7-葡萄糖苷、迷迭香酸、香草酸和松脂醇超过 1000.0 μ克/克甲醇提取物中。所讨论的提取物在磷钼、DPPH 和 ABTS 自由基清除以及 CUPRAC 和 FRAP 还原能力活性测定中显示出最高的活性(分别为 2.01、3.33、2.30、1.48 和 0.79 mg/ml)。水提取物在亚铁离子螯合试验中表现出最高的活性 (1.01 mg/ml)。虽然 EtOAc 提取物在乙酰胆碱酯酶 (AChE)、丁酰胆碱酯酶 (BChE) 和α-葡萄糖苷酶抑制活性测试中显示出高活性(分别为 1.11、1.49和 1.07 mg/ml),但 MeOH 提取物在酪氨酸酶和α-淀粉酶抑制活性测定(分别为 2.94 和 2.08 毫克/毫升)。提取物的总酚类/黄酮类化合物与其抗氧化活性之间存在高度相关性(相关系数超过 0.9)。此外,发现上述植物化学物质对抗氧化活性有显着贡献。结论是应该进行更详细的分析以确定负责提取物酶抑制活性的化合物。