Eosinophilic asthma is a form of bronchial asthma that is caused by the pulmonary infiltration of eosinophils and accounts for approximately half of the patients with severe asthma. Several cell types of the immune system in synergy with the epithelial cells of the lung provoke an inflammatory response in patients with asthma. Recently, the effect of fasting on immune cells and inflammation has attracted considerable attention. Therefore, we examined whether fasting may serve as novel preventive strategy in patients with asthma. In our study, we employed a previously established mouse model of eosinophilic asthma. C57BL/6 mice were inoculated intranasally with interleukin-33 and ovalbumin (OVA) in order to induce eosinophil infiltration in the lung and subjected to a 48-h long fasting period directly after or 7 days postinoculation. We used flow cytometry to characterise infiltrated immune cells in the lung and measured the quantity of inflammatory cytokines as well as antigen-specific immunoglobins (Ig) by ELISA. Our results indicated that fasting lowered the number of eosinophilic pulmonary infiltrates in the eosinophilic asthma model mice. Furthermore, fasting suppressed anti-OVA IgG1 production. Fasting suppressed Th2 cytokine production by impairing Th2 accumulation in the lung. The findings suggest that fasting may be a novel preventive strategy for eosinophilic asthma.

中文翻译：

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禁食损害嗜酸性哮喘小鼠模型肺部 2 型辅助 T 细胞浸润

嗜酸性粒细胞性哮喘是一种由嗜酸性粒细胞浸润肺部引起的支气管哮喘，约占重度哮喘患者的一半。免疫系统的几种细胞类型与肺上皮细胞协同作用会在哮喘患者中引发炎症反应。最近，禁食对免疫细胞和炎症的影响引起了相当大的关注。因此，我们检查了禁食是否可以作为哮喘患者的新预防策略。在我们的研究中，我们采用了先前建立的嗜酸性哮喘小鼠模型。C57BL/6 小鼠鼻内接种白细胞介素 33 和卵清蛋白 (OVA)，以诱导肺中的嗜酸性粒细胞浸润，并在接种后直接或 7 天后禁食 48 小时。我们使用流式细胞术来表征肺中浸润的免疫细胞，并通过 ELISA 测量炎性细胞因子和抗原特异性免疫球蛋白 (Ig) 的数量。我们的结果表明，禁食降低了嗜酸性哮喘模型小鼠中嗜酸性肺浸润的数量。此外，禁食抑制了抗 OVA IgG1 的产生。禁食通过损害肺部的 Th2 积累来抑制 Th2 细胞因子的产生。研究结果表明，禁食可能是一种新的嗜酸性哮喘预防策略。此外，禁食抑制了抗 OVA IgG1 的产生。禁食通过损害肺部的 Th2 积累来抑制 Th2 细胞因子的产生。研究结果表明，禁食可能是一种新的嗜酸性哮喘预防策略。此外，禁食抑制了抗 OVA IgG1 的产生。禁食通过损害肺部的 Th2 积累来抑制 Th2 细胞因子的产生。研究结果表明，禁食可能是一种新的嗜酸性哮喘预防策略。