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Dynamics of the urinary microbiome in pregnancy and the coincidental predictive value of the microbiota for IVF/IVF–ICSI outcome
Reproductive BioMedicine Online ( IF 4 ) Pub Date : 2021-08-06 , DOI: 10.1016/j.rbmo.2021.07.018
Rivka Koedooder 1 , Delshad M Maghdid 2 , Nicole G M Beckers 3 , Sam Schoenmakers 4 , Dik J Kok 2 , Joop S E Laven 1
Affiliation  

Research question

What is the impact of clinical pregnancy on the composition of the urinary microbiota?

Design

Eighty-five women receiving IVF, without or with intracytoplasmic sperm injection (ICSI) treatment were enrolled in a prospective observational study performed in 2008. Approximately 14 weeks before the start of hormonal treatment and embryo transfer, a midstream urine sample was obtained, followed by an additional sample 16 weeks after embryo transfer. The microbial composition was determined by polymerase chain reaction of the V1–V3 regions of the 16S rRNA bacterial gene. Clinical pregnancy data were collected after the first IVF/IVF–ICSI cycle and 1 year later.

Results

A significant decrease in the abundance of Lactobacillus species as well as a significant increase in that of Staphylococcus species was observed in women who became pregnant after IVF/IVF–ICSI treatment (both P < 0.0001). In addition, based on the composition of the pretreatment microbiome it was possible to identify women with a lower likelihood of achieving clinical pregnancy after IVF/IVF–ICSI treatment. The resulting prediction model was validated in another 27 women who did not become pregnant during the first cycle and received additional IVF/IVF–ICSI cycle(s) or frozen embryo transfer(s). The model predicted the women with no clinical pregnancy after IVF/IVF–ICSI treatment with a sensitivity of 0.42 and a specificity of 1.00.

Conclusions

The data primarily showed that clinical pregnancy results in significant changes in the abundance and diversity of the urinary microbiota. Coincidentally, it was discovered that the urinary microbiome composition before IVF/IVF–ICSI treatment can potentially be used as a predictor of clinical pregnancy.



中文翻译:

妊娠期尿微生物群动态和微生物群对 IVF/IVF-ICSI 结果的巧合预测价值

研究问题

临床妊娠对泌尿微生物群的组成有何影响?

设计

2008 年进行的一项前瞻性观察研究招募了 85 名接受体外受精(未接受或接受 ICSI)治疗的女性。在激素治疗和胚胎移植开始前大约 14 周,获得了中游尿液样本,然后是胚胎移植后 16 周再采集一份样本。通过 16S rRNA 细菌基因的 V1-V3 区域的聚合酶链反应确定微生物组成。在第一个 IVF/IVF-ICSI 周期后和 1 年后收集临床妊娠数据。

结果

在 IVF/IVF-ICSI 治疗后怀孕的女性中观察到乳酸菌物种丰度显着下降,葡萄球菌物种丰度显着增加(均P  < 0.0001)。此外,根据治疗前微生物组的组成,可以识别在 IVF/IVF-ICSI 治疗后临床妊娠可能性较低的女性。由此产生的预测模型在另外 27 名在第一个周期期间未怀孕并接受额外的 IVF/IVF-ICSI 周期或冷冻胚胎移植的女性中得到验证。该模型以 0.42 的敏感性和 1.00 的特异性预测 IVF/IVF-ICSI 治疗后没有临床妊娠的女性。

结论

数据主要表明临床妊娠导致尿液微生物群的丰度和多样性发生显着变化。巧合的是,人们发现 IVF/IVF-ICSI 治疗前的尿液微生物组组成可能被用作临床妊娠的预测指标。

更新日期:2021-08-06
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