Originally named according to their strict nucleophile specificity towards pyruvate, some pyruvate aldolases were demonstrated to be able to convert other nucleophiles. This study illustrates that the nucleophile 2-oxopentanoate successfully reacted with various aldehydes and even ketones. Unknown aldols with a 3S configuration were then isolated, characterized, and their stereochemistries could be set as either 4R or 4S by choosing the appropriate aldolase.

中文翻译：

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在探索 II 类丙酮酸醛缩酶亲核和亲电底物特异性方面向前迈进了一步

最初根据它们对丙酮酸的严格亲核特异性命名，一些丙酮酸醛缩酶被证明能够转化其他亲核试剂。这项研究表明亲核试剂 2-氧代戊酸酯成功地与各种醛甚至酮反应。然后分离、表征具有 3S 构型的未知醛醇，通过选择合适的醛缩酶，可以将它们的立体化学设置为 4 R或 4 S。