A low cost and effective indirect competitive method is reported to detect five EDCs, 17-beta-estradiol (E2), estriol (E3), bisphenol A (BPA), diethylstilbestrol (DES), and nonylphenol (NP) simultaneously, based on suspension array technology (SAT). Five kinds of complete antigens (E2-BSA, E3-BSA, BPA-BSA, DES-BPA, NP-BSA) were coupled to different encoding microspheres using purpose-made solutions in our laboratory instead of commercially available amino coupling kits; the method was further optimized for determination and reducing the cost. Encoding and signaling fluorescence of the particles are determined at 635/532 nm emission wavelengths. High-throughput curves of five EDCs were draw and the limit of detection (LOD) were between 0.0010 ng mL⁻¹ ~ 0.0070 ng mL⁻¹. Compared with traditional ELISA methods, the SAT exhibited better specificity and sensitivity. Experiments using spiked milk and tap water samples were also carried out, and the recovery was between 85 and 110%; the results also confirmed good repeatability and reproducibility. It illustrated great potential of the present strategy in the detection of EDCs in actual samples.

Graphical abstract

中文翻译：

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用于五种常见内分泌干扰物化学物质多重免疫测定的悬浮阵列

据报道，一种低成本且有效的间接竞争方法可基于悬浮液同时检测五种 EDC、17-β-雌二醇 (E2)、雌三醇 (E3)、双酚 A (BPA)、己烯雌酚 (DES) 和壬基酚 (NP)阵列技术（SAT）。5种完整抗原（E2-BSA、E3-BSA、BPA-BSA、DES-BPA、NP-BSA）使用我们实验室特制的溶液而不是市售的氨基偶联试剂盒与不同的编码微球偶联；进一步优化了测定方法，降低了成本。粒子的编码和信号荧光在 635/532 nm 发射波长处确定。绘制5种EDCs的高通量曲线，检测限（LOD）在0.0010 ng mL ^-1 ~ 0.0070 ng mL ^-1. 与传统的ELISA方法相比，SAT表现出更好的特异性和灵敏度。还进行了加标牛奶和自来水样品的实验，回收率在85%到110%之间；结果还证实了良好的重复性和再现性。它说明了本策略在检测实际样品中的 EDC 方面的巨大潜力。

图形概要