Kasamarda (C. occidentalis L.) is a traditional herb recently recognized as a potential nutraceutical in bone health. The current botanical nutraceutical regulations require consistent standardization for biological applications. The present study reported the standardization of bioactive flavonoid luteolin from Cassia occidentalis L. using validated high-performance thin-layer chromatographic (HPTLC) densitometric (DS) method. The mobile phase composition of toluene, ethyl acetate, and formic acid was optimized to separate and identify luteolin using silica gel 60F254 aluminum plates. The densitometric (DS) scanning was performed at 353 nm. This HPTLC-DS method was further validated as per ICH guidelines. The linearity was 200–700 ng/band with a correlation coefficient value of 0.994. The LOD and LOQ were found to be 54.06 ng/band and 163.84 ng/band, respectively. The recovery (88.38% and 100.72%) and precision (RSD,<5%) indicated method performance is robust and accurate for the routine analysis. Further, this bioactive flavonoid presence was confirmed and quantified by UV-spectrumin the sample matrix using this validated HPTLC-DS method. This HPTLC-DS method was robust, precise and accurate for quality control of active constituents present in C. occidentalis L.

中文翻译：

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在 Kasamarda (Cassia occidentalis L.) 中通过密度测定法鉴定和定量木犀草素的快速、灵敏的高效薄层色谱 (HPTLC) 方法

Kasamarda (C. occidentalis L.) 是一种传统草药，最近被认为是一种潜在的骨骼健康营养品。当前的植物营养食品法规要求对生物应用进行一致的标准化。本研究报告了使用经过验证的高性能薄层色谱 (HPTLC) 光密度 (DS) 方法对来自 Cassia occidentalis L. 的生物活性黄酮类木犀草素进行标准化。使用硅胶 60F254 铝板优化甲苯、乙酸乙酯和甲酸的流动相组成以分离和鉴定木犀草素。密度计 (DS) 扫描在 353 nm 处进行。这种 HPTLC-DS 方法根据 ICH 指南进行了进一步验证。线性为 200–700 ng/条带，相关系数值为 0.994。发现 LOD 和 LOQ 为 54.06 ng/条带和 163。分别为 84 ng/条带。回收率（88.38% 和 100.72%）和精密度（RSD，<5%）表明方法性能对于常规分析具有稳健性和准确性。此外，使用这种经过验证的 HPTLC-DS 方法，通过样品基质的紫外光谱确认和量化了这种生物活性黄酮类化合物的存在。这种 HPTLC-DS 方法对于 C. occidentalis L 中存在的活性成分的质量控制是稳健、精确和准确的。