Pseudomonas aeruginosa and Staphylococcus aureus causing common eye-infection ‘keratitis’ develop resistance to antibiotics by forming biofilm. Present study evaluates anti-biofilm properties of three commercially available eye care herbal formulations against P. aeruginosa and S. aureus. Eye drop formulations were tested for total phenolic content and antimicrobial activity. Biofilm growth over glass in the presence of herbal formulation was examined under fluorescence microscope. The herbal formulations rich in phenolics, tested at 10% (v/v) concentration showed growth inhibitory effect on planktonic cells. During biofilm mode of growth, 10% of herbal formulation caused considerable decline in growth of biofilm, although did not inhibit it completely. The response to different herbal formulations varied markedly among the different bacterial strains tested. Herbal components can significantly affect the surface topology of biofilms, thereby restricting the attachment of the cell to the substratum.

中文翻译：

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通过草药眼部护理配方控制细菌生物膜的进展

导致常见眼部感染“角膜炎”的铜绿假单胞菌和金黄色葡萄球菌通过形成生物膜对抗生素产生抗药性。本研究评估了三种市售的眼部护理草药制剂对铜绿假单胞菌和金黄色葡萄球菌的抗生物膜特性。测试滴眼剂制剂的总酚含量和抗微生物活性。在荧光显微镜下检查了在草药制剂存在下玻璃上的生物膜生长。富含酚类物质的草药配方在 10% (v/v) 浓度下测试显示对浮游细胞的生长抑制作用。在生物膜生长模式下，10%的草药制剂导致生物膜生长显着下降，但并未完全抑制它。对不同草药配方的反应在测试的不同细菌菌株中显着不同。草药成分可以显着影响生物膜的表面拓扑结构，从而限制细胞对基质的附着。