Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, a high mortality disease. The development of such disease depends on the interaction of fungal cells with macrophages, in which they can reside and replicate. In order to dissect the molecular mechanisms by which cryptococcal cells modulate the activity of macrophages, a genome-scale comparative analysis of transcriptional changes in macrophages exposed to Cryptococcus spp. was conducted. Altered expression of nearly 40 genes was detected in macrophages exposed to cryptococcal cells. The major processes were associated with the mTOR pathway, whose associated genes exhibited decreased expression in macrophages incubated with cryptococcal cells. Phosphorylation of p70S6K and GSK-3β was also decreased in macrophages incubated with fungal cells. In this way, Cryptococci presence could drive the modulation of mTOR pathway in macrophages possibly to increase the survival of the pathogen.

中文翻译：

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转录组分析表明，巨噬细胞中的 mTOR 通路可以通过隐球菌细胞的存在进行调节。

新型隐球菌和格特隐球菌是隐球菌病（一种高死亡率疾病）的病原体。这种疾病的发展取决于真菌细胞与巨噬细胞的相互作用，真菌细胞可以在巨噬细胞中驻留和复制。为了剖析隐球菌细胞调节巨噬细胞活性的分子机制，对暴露于隐球菌属的巨噬细胞的转录变化进行了基因组规模的比较分析。进行了。在暴露于隐球菌细胞的巨噬细胞中检测到近 40 个基因的表达发生改变。主要过程与 mTOR 通路相关，其相关基因在与隐球菌细胞一起孵育的巨噬细胞中表现出表达降低。在与真菌细胞一起孵育的巨噬细胞中，p70S6K 和 GSK-3β 的磷酸化也有所降低。通过这种方式，隐球菌的存在可以驱动巨噬细胞中 mTOR 通路的调节，从而可能增加病原体的存活率。