Wounds may remain open for a few weeks in severe burns, which provide an entry point for pathogens and microorganisms invading. Thus, wound dressings with long-term antimicrobial activity are crucial for severe burn wound healing. Here, a sandwich structure composite wound dressing anchored with silver nanoparticles (AgNPs) was developed for severe burn wound healing. AgNPs were in situ synthesized on the fibers of chitosan nonwoven fabric (CSNWF) as the interlayer of wound dressing for sustained release of silver ion. The firmly anchored AgNPs could prevent its entry into the body, thereby eliminating the toxicity of nanomaterials. The outer layer was a polyurethane membrane, which has a nanoporous structure that could maintain free transmission of water vapor. Chitosan/collagen sponge was selected as the inner layer because of its excellent biocompatibility and biodegradability. The presence of AgNPs in the CSNWF was fully characterized, and the high antibacterial activity of CSNWF/AgNPs was confirmed by against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The superior wound healing effect on deep dermal burns of presented composite wound dressing was demonstrated in a porcine model. Our finding suggested that the prepared AgNPs doped sandwich structure composite wound dressing has great potential application in severe wound care.

中文翻译：

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一种夹层结构复合伤口敷料，具有牢固锚定的银纳米粒子，用于猪模型的严重烧伤伤口愈合

在严重烧伤中，伤口可能会保持开放数周，这为病原体和微生物的入侵提供了切入点。因此，具有长期抗菌活性的伤口敷料对于严重烧伤伤口的愈合至关重要。在这里，开发了一种以银纳米粒子 (AgNPs) 锚定的夹层结构复合伤口敷料，用于严重烧伤伤口的愈合。AgNPs在壳聚糖无纺布（CSNWF）的纤维上原位合成，作为伤口敷料的中间层，用于缓释银离子。牢固锚定的AgNPs可以防止其进入体内，从而消除纳米材料的毒性。外层是聚氨酯膜，它具有纳米孔结构，可以保持水蒸气的自由传输。选择壳聚糖/胶原蛋白海绵作为内层是因为其具有优异的生物相容性和生物降解性。CSNWF中AgNPs的存在得到了充分的表征，CSNWF/AgNPs对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抗菌活性得到了证实。在猪模型中证明了所提出的复合伤口敷料对深部皮肤烧伤的卓越伤口愈合效果。我们的研究结果表明，制备的AgNPs掺杂夹层结构复合伤口敷料在严重伤口护理中具有巨大的应用潜力。在猪模型中证明了所提出的复合伤口敷料对深部皮肤烧伤的卓越伤口愈合效果。我们的研究结果表明，制备的AgNPs掺杂夹层结构复合伤口敷料在严重伤口护理中具有巨大的应用潜力。在猪模型中证明了所提出的复合伤口敷料对深部皮肤烧伤的卓越伤口愈合效果。我们的研究结果表明，制备的AgNPs掺杂夹层结构复合伤口敷料在严重伤口护理中具有巨大的应用潜力。